Research reportIsolation and characterization of highly purified rat intestinal intraepithelial lymphocytes
References (26)
- et al.
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Anal. Biochem.
(1987) - et al.
Pitfalls in the characterization of small intestinal lymphocytes
J. Immunol. Methods
(1995) - et al.
Isolation of functionally active intraepithelial lymphocytes and enterocytes from human small and large intestine
J. Immunol. Methods
(1992) - et al.
Oligoclonality and skewed T cell receptor Vβ gene segment expression in in vivo activated human intestinal intraepithelial T lymphocytes
Immunobiology
(1994) - et al.
Differential function of intestinal intraepithelial lymphocyte subsets
J. Immunol.
(1992) - et al.
Differences in intraepithelial lymphocyte T cell subsets isolated from murine small versus large intestine
J. Immunol.
(1995) - et al.
Expression and regulation of adhesion molecules by γδ T cells from lymphoid tissues and intestinal epithelium
Eur. J. Immunol.
(1994) - et al.
Human colonic intraepithelial and lamina proprial lymphocytes: cytotoxicity in vitro and the potential effects of the isolation method on their functional properties
Gut
(1981) - et al.
Preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine
Gut
(1981) - et al.
Interleukin 1 receptor antagonist is a member of the interleukin 1 gene family: Evolution of a cytokine control mechanism
Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor α/β+/CD2− cells and high expression of the RT6 alloantigen
Eur. J. Immunol.
Function of αβ TCR+ intestinal intraepithelial lymphocytes: Th1- and Th2-type cytokine production by CD4+ CD8− and CD4+ CD8+ T cells for helper activity
Int. Immunol.
αβ T cell receptor-positive intraepithelial lymphocytes with CD4+, CD8− and CD4+, CD8+ phenotypes from orally immunized mice provide Th2-like function for B cell responses
J. Immunol.
Cited by (51)
Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms
2021, PhytomedicineCitation Excerpt :Similarly, the recovery rates of MTL, SS, and SP in the serum of SDS rats by SJZD and the recovery rate of GI hormones in the serum of SDS rats by S-3 and NPS were calculated. Intraepithelial lymphocytes (IELs) were prepared according to a modification of the method of Dina Montufar-Solis et al. (James et al., 2020; Kearsey & Stadnyk, 1996). Briefly, the small intestine was cut into 5-mm pieces in a 100 mm tissue culture dish supplemented with RPMI-1640 with 1% double-antibiotics (penicillin-streptomycin) and 10% fetal bovine serum (FBS).
Optimization of murine small intestine leukocyte isolation for global immune phenotype analysis
2014, Journal of Immunological MethodsCitation Excerpt :Unfortunately, comparison of specific frequencies between studies proved difficult because previous studies reported percentages of total lymphocytes rather than total intestinal cells. Such difficulties comparing results between gut immune cell phenotype studies that utilize different isolation protocols has been a topic of discussion for some time (Van der Heijden and Stok, 1987; Kearsey and Stadnyk, 1996). Indeed, the current study demonstrates that significant differences can be obtained simply by altering the isolation protocol (Fig. 4).
Involvement of intestinal intraepithelial lymphocytes in turnover of intestinal epithelial cells: Morphological and functional alterations due to daily administration of FK506
2012, Cellular ImmunologyCitation Excerpt :P815, a mouse mastcytoma cell line, was cultured at 37 °C with RPMI-1640 medium supplemented with heat-inactivated 10% fetal bovine serum (ICN Biomedicals). IECs and IELs were isolated and prepared according to a modification of a previously published method [28]. The resected intestines (from pylorus to ileum) were cut into 2–3 cm segments and inverted.
Chitosan enhances transcellular permeability in human and rat intestine epithelium
2012, European Journal of Pharmaceutics and BiopharmaceuticsSignals elicited at the intestinal epithelium upon chitosan feeding contribute to immunomodulatory activity and biocompatibility of the polysaccharide
2010, VaccineCitation Excerpt :In the afternoon we administered 0.1 M acetic acid (diluent group) or acetic acid containing 1, 3 or 5 mg chitosan in a final volume of 200 μl per os by using a plastic pipette tip. Sixteen hours later [17,18], we obtained the small gut, we strictly removed and discarded all PPs and we purified IECs according to standard procedures [19]. Briefly, the small gut was rinsed in Ca2+ and Mg2+ free PBS and washed in PBS-dithiothreitol (200 μg/ml) to eliminate residual mucus and then shaken in RPMI 1640 (Sigma–Aldrich, Saint Louis, MO, USA) containing 0.03 M EDTA to remove the epithelium at 37 °C [16].
Quantitative isolation of mouse and human intestinal intraepithelial lymphocytes by elutriation centrifugation
2009, Journal of Immunological Methods