Cell
Volume 38, Issue 1, August 1984, Pages 265-273
Journal home page for Cell

Article
DNA sequences required for regulated expression of β-globin genes in murine erythroleukemia cells

https://doi.org/10.1016/0092-8674(84)90548-8Get rights and content

Abstract

We introduced into MEL cells rabbit β-globin gene deletion mutants and two sets of hybrid genes constructed from the inducible human β-globin gene and noninducible human γ-globin gene or the murine H-2Kbm1 class I MHC gene. S1 nuclease analysis of gene transcripts before and after MEL differentiation showed that induction of the rabbit β-globin gene did not require more than 58 bp of DNA 5′ to the transcription initiation site. Hybrid genes were constructed with human β-globin DNA sequences from either 5′ or 3′ of the translation initiation site linked to the complementary parts of the γ or H2Kbm1 genes. Both types of constructs were inducible during MEL differentiation. The relative rates of transcription of the 5′γ-3′β and 5′H2-3′ β hybrid genes show that induction of the hybrid gene transcripts results at least in part from transcriptional activation of the genes. We suggest that DNA sequences that regulate β-globin gene transcription during MEL differentiation are located both 5′ and 3′ to the translation initiation site.

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