Cell
Volume 41, Issue 1, May 1985, Pages 31-40
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Article
A novel ras-related gene family

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Abstract

We have identified a new family of ras genes, the rho genes, which share several properties with the more classical ras gene family consisting of N-, K-, and H-ras. The rho genes, first isolated from a cDNA library from the abdominal ganglia of Aplysia, encode proteins that share 35% amino acid homology with H-ras. Evolutionarily conserved counterparts of rho have been detected in yeast, in Drosophila, in rat, and in man. Sequence analysis reveals over 85% homology between the human and Aplysia proteins. The ras and rho gene products share several common properties; both are 21,000 daltons, both reveal C-terminal sequences required for membrane attachment, and both show blocks of strong internal homology, suggesting that the two proteins may share common functions but may use these functions in different ways.

References (49)

  • B.M. Sefton et al.

    The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus, and Abelson leukemia virus contain tightly bound lipid

    Cell

    (1982)
  • E. Taparowsky et al.

    Structure and activation of the human N-ras gene

    Cell

    (1983)
  • T. Toda et al.

    In yeast, RAS proteins are controlling elements of adenylate cyclase

    Cell

    (1985)
  • J. Vieira et al.

    The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

    Gene

    (1982)
  • M.C. Willingham et al.

    Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry

    Cell

    (1980)
  • K. Zinn et al.

    Identification of two distinct regulatory regions adjacent to the human β-interferon gene

    Cell

    (1983)
  • E.H. Chang et al.

    Tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of Harvey murine sarcoma virus

    Nature

    (1982)
  • J. Chirgwin et al.

    Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

    Biochemistry

    (1979)
  • D. Defeo-Jones et al.

    ras related gene sequences identified and isolated from Saccharomyces cerevisiae

    Nature

    (1983)
  • R.F. Doolittle

    Similar amino acid sequences: chance or common ancestry?

    Science

    (1981)
  • R.W. Ellis et al.

    The viral and cellular p21 (ras) gene family

  • R. Ellis et al.

    The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes

    Nature

    (1981)
  • O. Fasano et al.

    Analysis of the transforming potential of the human H-ras gene by random mutagenesis

  • J. Fiddes et al.

    isolation, cloning and sequence analysis of the cDNA for the alpha subunit of human chorionic gonadotropin

    Nature

    (1979)
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