Cell
Volume 79, Issue 5, 2 December 1994, Pages 791-803
Journal home page for Cell

Article
Overexpression of cadherins and underexpression of β-catenin inhibit dorsal mesoderm induction in early Xenopus embryos

https://doi.org/10.1016/0092-8674(94)90069-8Get rights and content

Abstract

The cadherin-catenin complex has an important role in cell-cell adhesion and may also function in signaling pathways. We report that overexpression of three cadherin types in Xenopus embryos causes them to develop with reduced dorsal axial structures. The same phenotype is produced in embryos that have been depleted of maternal β-catenin protein by an antisense oligodeoxynucleotide complementary to β-catenin mRNA. They show an inhibition in the expression of dorsal mesodermal markers MyoD and goosecoid, but not of ventral and general mesodermal markers. They lack notochords, somites, and neural tubes and are defective in dorsal mesodermal signaling in Nieuwkoop assays. The phenotype can be rescued by the injection of β-catenin mRNA and not by the injection of Xwnt-8 mRNA. These results show that β-catenin has an important role in dorsal mesoderm induction. They directly demonstrate the activity of a maternal mRNA in axis specification.

References (54)

  • P.A. Krieg et al.

    In vitro RNA synthesis with SP6 RNA polymerase

    Meth. Enzymol.

    (1987)
  • M. Peifer et al.

    Knowing your neighbors: cell interactions determine intrasegmental pattern in Drosophila

    Trends Genet.

    (1992)
  • S. Sokol et al.

    Injected Wnt RNA induces a complete body axis in Xenopus embryos

    Cell

    (1991)
  • E. Wieschaus et al.

    Autonomous requirements for the segment polarity gene armadillo during Drosophila oogenesis

    Cell

    (1987)
  • S.L. Wolda et al.

    Overlapping expression of Xwnt 3a and Xwnt 1 in neural tissue of X. laevis embryos

    Dev. Biol.

    (1993)
  • E. Amaya et al.

    FGF signalling in the early specification of mesoderm in Xenopus

    Development

    (1993)
  • B. Angres et al.

    Differential expression of two cadherins in Xenopus laevis

    Development

    (1991)
  • M. Beckerle

    Identification of a new protein localized at sites of cell substratum adhesion

    J. Cell Biol.

    (1986)
  • R.S. Bradley et al.

    Expression of Wnt1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion

    J. Cell Biol.

    (1993)
  • A. Chakrabati et al.

    Secretory and inductive properties of Drosophila wingless protein in Xenopus oocytes and embryos

    Development

    (1992)
  • Y.S. Choi et al.

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm

    J. Cell Biol.

    (1989)
  • Y.S. Choi et al.

    A cadherin-like protein in eggs and cleaving embryos of X. laevis is expressed in oocytes in response to progesterone

    J. Cell Biol.

    (1990)
  • J. Christian et al.

    Xwnt-8, a Xenopus Wnt1int-related gene responsive to mesoderm inducing factors may play a role in ventral mesoderm patterning during embryogenesis

    Development

    (1991)
  • T. Fujimori et al.

    Ectopic expression of N-cadherin perturbs histogenesis in Xenopus embryos

    Development

    (1990)
  • B. Geiger et al.

    Broad spectrum pan-cadherin antibodies, reactive with the C terminal 24 amino acid residues of N-cadherin

    J. Cell Sci.

    (1990)
  • J. Gerhart et al.

    Cortical rotation of the Xenopus egg: consequences for the anteroposterior pattern of embryonic dorsal development

    Development (Suppl.)

    (1989)
  • D. Ginsberg et al.

    Expression of a novel cadherin (EP-cadherin) in unfertilized eggs and early Xenopus embryos

    Development

    (1991)
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