Chemical coding of neurons that project from different regions of intestine to the coeliac ganglion of the guinea pig

doi:10.1016/0165-1838(95)00053-1

Journal of the Autonomic Nervous System

Volume 56, Issues 1–2, 5 December 1995, Pages 15-25

Journal of the Auton...

https://doi.org/10.1016/0165-1838(95)00053-1 Get rights and content

Abstract

The chemical codings of neurons that project from the small intestine, caecum, proximal colon, distal colon and rectum to the coeliac ganglion of the guinea pig were investigated. The coeliac ganglion was injected with the retrogradely transported dye Fast Blue, and each of the regions was examined 6 days later in wholemounts that had been prepared for immunohistochemical localisation of pairs of antigens. In both the small and large intestines, all intestinofugal neurons were immunoreactive (IR) for choline acetyltransferase (ChAT). In each region of the large intestine, the largest population, representing 50–60% of retrogradely labelled neurons in each region, was immunoreactive for ChAT, bombesin (BN), calbindin (Calb) and nitric oxide synthase (NOS). Most intestinofugal neurons in the small intestine contain bombesin and VIP-IR along with ChAT-IR but none contain either Calb or NOS. Thus, nerve endings of enteric origin in the coeliac ganglion that contain NOS-IR or Calb-IR come from the large intestine and those with bombesin-IR but not NOS-IR are from the small intestine. The gastric wall was injected with Fast Blue in order to label noradrenergic (NA) neurons in the coeliac ganglion and to determine, by localisation of NOS and bombesin-IR, whether they receive inputs from the small and large intestine. Some NA neurons received inputs from the large intestine (and perhaps also from the small intestine) and some received inputs exclusively from the small intestine. Most NA neurons that received intestinofugal inputs had the chemical code NA/−; some were immunoreactive for somatostatin ( $NA SOM$ neurons), but those with IR for neuropeptide Y ( $NA NPY$ ) rarely received intestinofugal inputs.

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Further, cholinergic innervation of the celiac ganglion may not necessarily be vagal. Viscerofugal neurons from the gastrointestinal tract traveling cranially to synapse the celiac ganglion have been reported in the literature (Domoto et al., 1995; Furness et al., 2000; Messenger and Furness, 1992), as well as their cholinergic phenotype (Furness et al., 2000; Lomax et al., 2000; Mann et al., 1995). These studies support the notion that the celiac ganglion is part of the abdominal feedback that modulates reflex inputs from different parts of the digestive tract (Frantzides et al., 1987; King and Szurszewski, 1989; Semba, 1954).
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Research paperChemical coding of neurons that project from different regions of intestine to the coeliac ganglion of the guinea pig

Abstract

J. Auton. Nerv. Syst.

J. Auton. Nerv. Syst.

Neuroscience

Neuropharmacology

Gastroenterology

J. Auton. Nerv. Syst.

Brain Res.

J. Auton. Nerv. Syst.

J. Auton. Nerv. Syst.

Neuroscience

Brain Res.

J. Auton. Nerv. Syst.

J. Auton. Nerv. Syst.

Nitric oxide synthase-containing neurons in the pig large intestine: topogrraphy, morphology, and viscerofugal projections

Microsc. Res. Tech.

Antibodies raised against synthetic peptides react with choline acetyltransferase in various immunoassay and in immunohistochemistry

J. Neurochem.

An immunocytochemical investigation with monoclonal antibodies to somatostatin

Histochemistry

Excitatory input from the distal colon to the inferior mesenteric ganglion in the guinea-pig

J. Physiol.

Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer

Nature

Origins of nerve terminals containing nitric oxide synthase in the guinea-pig coeliac ganglion

J. Auton. Nerv. Syst.

Immunohistochemical evidence for the presence of calcium binding proteins in enteric neurons

Cell Tissue Res.

Projections of neurons with neuromedin U-like immunoreactivity in the small intestine of the guinea-pig

Cell Tissue Res.

Research paper
Chemical coding of neurons that project from different regions of intestine to the coeliac ganglion of the guinea pig