Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies

doi:10.1016/0270-9139(95)90578-2

Hepatology

Volume 22, Issue 2, August 1995, Pages 546-552

https://doi.org/10.1016/0270-9139(95)90578-2 Get rights and content

Abstract

Primary pig hepatocytes form a tissuelike structure in an in vitro culture model that has provision for three-dimensional cell orientation, cell aggregation, decentralized cell perfusion with low metabolite gradients, integral oxygenation, and nonparenchymal cell coculture. Scanning electron microscopy (SEM) has shown that hepatocytes spontaneously form aggregates in a three-dimensional structure between and on the surface of artificial capillaries. Transmission electron microscopy (TEM) has shown that after 7 weeks of in vitro perfusion, the cell ultrastructure remains similar to that of the parenchyma in vivo. Golgi complexes, active membrane processes, reorganization of cell junctions, and bile canaliculi-like intercellular spaces were demonstrated.

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Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies

Abstract

Exp Cell Res

Exp Cell Res

Proteoglycans and glycosaminoglycans induce gap junction synthesis and function in primary liver cultures

J Cell Biol

Culturing hepatocytes and other differentiated cells

Hepatology

Reversal of fulminant hepatic failure using an extracorporeal liver assist device

Hepatology

New method of hepatocyte transplantation and extracorporal liver support

Ann Surg

Performance of a new hybrid artificial liver support system using hepatocytes entrapped within a hydrogel

ASAIO Trans

Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration

FASEB J

Evaluation of a hepatocyte entrapment hollow fiber bioreactor: a potential artificial liver

Biot Bioeng

Ex vivo gene therapy of familial hypercholesterolemia

Hum Gene Ther

Hepatocyte culture systems for artificial liver support: implications for critical care medicine (bioartificial liver support)

Crit Care Med

Acute liver failure

N Engl J Med

Isolated hepatocytes: preparation, properties and applications

Hepatocyte culture between three dimensional arranged biomatrix-coated independent artificial capillary systems and sinusoidal endothelial cell co-culture compartments

Artif Org

Culture model for primary hepatocytes

In Vitro Cell Dev Biol