Morphology of hepatocellular neoplasms in B6C3F1 mice

doi:10.1016/0304-3835(80)90024-5

Cancer Letters

Volume 9, Issue 4, June 1980, Pages 319-325

https://doi.org/10.1016/0304-3835(80)90024-5 Get rights and content

Abstract

The morphology and behavior of hepatocellular neoplasms in control B6C3F1 mice were studied and classified according to cell size, tinctorial properties of the cytoplasm, pattern of growth, and other morphological features. The small tumors were composed primarily of basophilic hepatocytes which grew in a solid adenomatous pattern. Larger solid tumors had foci of prominent trabecular formations. All mice with metastatic hepatocellular neoplasms had large areas of prominent trabecular formations in the primary liver tumors. It appears that trabecular hepatocellular carcinomas develop from adenomatous neoplasms in the liver of B6C3F1 mice. The histogenesis and morphology of hepatocellular neoplasms in B6C3F1 mice is compared with those induced by carcinogens and differences are noted.

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The long-term toxicity/carcinogenicity of musk xylol, a synthetic nitro musk, was examined in B6C3F₁ mice of both sexes. Musk xylol was administered at dietary levels of 0 (control), 0.075 or 0.15% for 80 wk. The overall tumour incidences in all treated groups of both sexes were significantly higher than those in the corresponding controls. Combined malignant and benign liver cell tumours were clearly increased in both sexes, and in males a positive significant trend was also noted for the occurrence of hepatocellular carcinomas. In males the incidence of Harderian gland tumours was also significantly greater in treated groups than in controls. Some other neoplasms, such as lung tumours in both sexes and Harderian gland tumours and lymphomas in females, occurred in greater numbers in the treated groups, although the differences were not statistically significant in comparison with the controls. In addition, the incidences and total numbers of malignant tumours were significantly increased in treated groups of both sexes, although the increases were not dose dependent. The results demonstrated that musk xylol is carcinogenic in B6C3F₁ mice when given at dose levels of 0.075 or 0.15% in the diet for 80 wk.

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Research letterMorphology of hepatocellular neoplasms in B6C3F1 mice

Abstract

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Hepatic cytologic and neoplastic changed in mice given benzidine dihydrochloride

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J. Natl. Cancer Inst.

Morphology of spontaneous and induced neoplasia

Research letter
Morphology of hepatocellular neoplasms in B6C3F1 mice

α-Fetoprotein levels and hepatic alterations during chemical carcinogenesis in $C57BL 6N$ mice

Histochemistry and spontaneous hepatomas of $CBA H-T6T6$ mice. Histochemistry and electron microscopy