Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: Preliminary findings

Abstract

In the present study a methodological approach is taken which quantitates aberrant dysplastic crypts in the unsectioned murine colon. $\text{C57BL}\text{6J}$ or CF1 female mice (7–8 weeks old) were injected (i.p.) with azoxymethane (5 mg/kg body wt./week) for 4 weeks. Their colons were excised, cut open on the median axis and fixed flat in buffered formalin. Unsectioned colons were stained with methylene blue. The mucosal side was examined under a light microscope. The aberrant crypts, which are larger and have a thicker epithelial lining, were easily visualized using ×4 or ×10 objectives. CF1 mice, which are more sensitive to developing colon tumors, had a higher number of aberrant crypts/colon than their less sensitive counterparts, $\text{C57BL}\text{6J}$ mice (5.0 ± 0.7 vs. 2.4 ± 0.7). The usefulness of this observation as a possible measure of neoplastic events is discussed in the animal and human situation.