A high-resolution cytogenetic map of human chromosome 3: Localization of 291 new cosmid markers by direct R-banding fluorescence in situ hybridization

doi:10.1016/0888-7543(92)90018-N

Genomics

Volume 13, Issue 4, August 1992, Pages 1047-1055

https://doi.org/10.1016/0888-7543(92)90018-N Get rights and content

Abstract

We localized 291 new cosmid markers (including 65 RFLPs) on human chromosome 3 by direct R-banding fluorescence in situ hybridization. This system, which is based on fluorescence in situ hybridization combined with replicated prometaphase R-bands, allows the direct visualization of signals on R-banded prometaphases stained with propidium iodide and provides a more rapid and efficient method for genome mapping of cosmid clones. The signals of 291 markers examined here were localized preferentially to R-positive bands throughout chromosome 3. The detailed map positions of 366 clones and the characterization of 142 RFLPs, including the preliminary data reported by Yamakawa et al. (1991, Genomics 9: 536–543; and 11: 565–572), are summarized. This high-resolution cytogenetic map (average distance of 0.58 Mb), in conjunction with a genetic linkage map, can facilitate the analysis of chromosomal and molecular aberrations in genetic diseases and cancers. Furthermore, these mapping data will provide many useful landmarks for the construction of contig maps of chromosome 3.

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A high-resolution cytogenetic map of human chromosome 3: Localization of 291 new cosmid markers by direct R-banding fluorescence in situ hybridization

Abstract

Genomics

Genomics

Genomics

Genomics

Cell

Genomics

Genomics

Cell

Genomics

Genomics

Genomics

Cancer Genet. Cytogenet

Cancer Genet. Cytogenet

Cancer Genet. Cytogenet

Cancer Genet. Cytogenet

Cancer Genet. Cytogenet

Genomics

Genomics

Localization of polymorphic DNA probes frequently deleted in lung carcinoma

Hum. Genet

The gene for generalized thyroid hormone resistance (GTHR) is tightly linked to and may be identical with the proto-oncogene, c-erbA-beta

J. Hum. Genet

Deletion mapping in human renal cell carcinoma

Cancer Res

Molecular mapping of deletion sites in the short arm of chromosome 3 in human lung cancer

Genes Chrom. Cancer

Hereditary renal-cell carcinoma associated with a chromosomal translocation

N. Engl. J. Med

High-resolution R- and G-banding on the same preparation

Hum. Genet

Autosomal dominant retinis pigmentosa: Linkage to phodopsin and evidence for genetic heterogeneity

Genomics

Regional localization of chromosome 3-specific DNA fragments by using a hybrid cell deletion panel

Am. J. Hum. Genet

Regional assignment of the human thymidylate synthase (TS) gene to chromosome band 18p11.32 by nonisotopic in situ hybridization

Hum. Genet