Biochemical and Biophysical Research Communications
Complementary DNA cloning and sequencing of rat ovarian basic fibroblast growth factor and tissue distribution study of its mRNA
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Transcriptional and translational dual-regulated oncolytic herpes simplex virus type 1 for targeting prostate tumors
2010, Molecular TherapyCitation Excerpt :Construction of recombinant virus. The ARR2PB-ICP27 (A27) and ARR2PB-5′UTR-ICP27 (AU27) recombinant viruses were generated by the recombineering method,26,27 which replaced the endogenous ICP27 promoter with the ARR2PB ± promoter the 5′UTR (619 bp) of rat FGF-2 gene28 in BAC.p25 (Figure 1). A 538-bp region between UL53 and ICP27(UL54) was deleted to insert the promoter ± 5′UTR.
Nicotine-induced FGF-2 mRNA in rat brain is preserved during aging
2004, Neurobiology of AgingTranslational Thermotolerance Provided by Small Heat Shock Proteins Is Limited to Cap-dependent Initiation and Inhibited by 2-Aminopurine
2003, Journal of Biological ChemistryCitation Excerpt :For the pHsp-Cap-Luc, the Drosophila melanogaster Hsp70 promoter was excised from the pBN247 construct (42) using HindIII blunt/SalI and cloned in front of the luciferase gene using SmaI and XhoI sites from the vector. For the pHsp-IRES-Luc construct, the rat FGF-2 IRES (532 bp) was excised from pRObFGF503 (43) using HindIII/NcoI and inserted between the Hsp70 promoter and the luciferase reporter gene. Rat αB-crystallin cDNA was cloned NcoI-XhoI/SalI into β-actin vector (44).
The effects of repeated minimal electroconvulsive shock exposure on levels of mRNA encoding fibroblast growth factor-2 and nerve growth factor in limbic regions
2002, NeuroscienceCitation Excerpt :Antisense cRNA probes were synthesized from linearized plasmid templates by in vitro transcription with the appropriate (see below) RNA polymerase using [α-32P]CTP (3000 Ci/mmol, NEN) and MAXIScript in vitro transcription kit (Ambion) according to the manufacturer’s protocol, and as previously reported (Follesa et al., 1994). The plasmid RObFGF103 (a gift from Dr. Baird, Ciblex Corp., San Diego, CA, USA) is a derivative of a Bluescript plasmid containing a 1016-base portion of the rat FGF-2 (Shimasaki et al., 1988). NcoI-linearized plasmid was used as a template for the in vitro transcription with T7 RNA polymerase to generate a 524-base probe which includes 477 bases of FGF-2 and 47 bases of the Bluescript polylinker region.