Liver, Pancreas, and Biliary TractIdentification, culture, and characterization of pancreatic stellate cells in rats and humans☆,☆☆
Section snippets
Reagents
Materials were purchased from the following sources: collagenase I, rabbit antilaminin, retinol, retinyl-acetate, retinyl-palmitate, yeast transfer RNA, sonicated human placenta DNA, and monoclonal anti–c-fibronectin from Sigma Chemical Co. (Munich, Germany); OptiPrep from Nycomed Pharma (Oslo, Norway); Ficoll and polyU-homopolymer from Pharmacia LKB (Freiburg, Germany); horseradish peroxidase (HRP)–anti-mouse, HRP–anti-rabbit, fluorescein-conjugated streptavidin, biotinylated rabbit anti-mouse
Demonstration of PSCs in the normal and fibrotic pancreas of rats and humans
Cells with cytoplasmic lipid droplets were occasionally visible in the interlobular area or in the interacinar region (Figure 1). These cells often show a triangular shape, a prominent endoplasmic
Discussion
In this study, retinoid-containing fat-storing cells were identified in the pancreas of rats and humans. The cells contained numerous retinoid-containing fat droplets and were located in the interlobular and interacinar region. Because of their similarity to HSCs, we named them PSCs. The number of the cells increased with age. In addition, these cells are abundant in alcoholic chronic pancreatitis in humans. For further characterization, cells were obtained by outgrowth from small pancreas
Acknowledgements
The authors thank Martina Ehnle, Erika Schmidt, and Adolf Reich for expert technical assistance; Elke Wolff-Hieber for electron- microscopic photographs; and Deedee Falls for proofreading the manuscript.
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Address requests for reprints to: Max G. Bachem, M.D., Universität Ulm, Klinikum, Institut für Klinische Chemie, Bereichslabor Michelsberg, 89070 Ulm, Germany. e-mail: [email protected]; fax: (49)731-502-6719.
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Supported by grant Bausteinförderung P. 347 from the University of Ulm (to M.G.B.) and grant Kn 200/4-1 from the Deutsche Forschungsgemeinschaft (to G.A.).