Alimentary TractLymphocytes in the human gastric mucosa during Helicobacter pylori have a T helper cell 1 phenotype☆,☆☆
Section snippets
Subject population
Biopsy specimens of the gastric antrum were obtained from consenting subjects aged 20–55 years undergoing gastroesophageal duodenoscopy for various clinical indications as approved by our respective institutional review boards at Baylor College of Medicine and the University of Texas Medical Branch. Individuals regularly using nonsteroidal anti-inflammatory drugs or antisecretory drugs were excluded from the donor population. Subjects were considered infected if H. pylori was detected either by
Characterization of gastric T cells in situ and after isolation
To ensure that preparations of isolated gastric T cells were generally representative of the T-cell population in situ, immunohistochemistry for CD3, CD4, and CD8 was performed on frozen sections of antral biopsy specimens obtained from infected and uninfected individuals. Whereas staining with the isotype control did not mark any cells, specific antibodies showed that infection with H. pylori is associated with a marked T-cell infiltrate in the epithelium as well as in the lamina propria
Discussion
Previous studies have examined the ability of H. pylori to stimulate IFN-γ production and have described the presence of IFN-γ–producing cells in gastric tissue.26, 27, 28 However, the IFN-γ–producing cells obtained immediately after isolation from the gastric mucosa have not been characterized in detail. The data in this report support the hypothesis that the gastric T-cell population resembles the Th1 subset of helper T cells. Moreover, these cells are relatively restricted in their cytokine
Acknowledgements
The authors thank the endoscopy staff at the University of Texas Medical Branch and the Veterans Administration Hospital in Houston for their help in collecting the biopsy specimens. The technical assistance of Jennifer Graham and the staff in the histology service of the Department of Pathology at the University of Texas Medical Branch is also appreciated. Mark Griffin provided assistance in the development of the four-color flow-cytometric analysis and the evaluation of the data.
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2022, Current Opinion in ImmunologyCitation Excerpt :Accordingly, H. pylori infection is associated with enhanced induction and recruitment of regulatory T cells (Tregs) to the mucosa [6,7]. Despite the vast array of immune-evasion strategies, numerous studies revealed that H. pylori infection also provokes the induction of Th1 and Th17 effector cells as well as their signature cytokines, IFNγ and IL-17, respectively [8–14]. However, the immune response elicited by H. pylori fails to clear the pathogen and the resulting persistent infection triggers inflammatory processes with detrimental consequences for the host.
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Address requests for reprints to: Peter B. Ernst, Children's Hospital, University of Texas Medical Branch, 301 University Boulevard, Galveston, Texas 77555-0366. e-mail: [email protected]; fax: (409) 772-1761.
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Supported by the John Sealy Memorial Endowment Fund for Biomedical Research and U.S. Public Health Service grants DK 50669, DK 51577, and CHD 35741.