Proceedings of the XVIIth World Congress of the Transplantation SocietyImmunomodulation of intestinal transplant with allograft irradiation and simultaneous donor bone marrow infusion
Section snippets
Methods
Inbred male Lewis (LEW, RT11) and Brown Norway (BN, RT1n) rats were purchased from Harlan Sprague Dawley, Inc., (Indianapolis, IN) and used as donors and recipients, respectively. Small intestinal grafts were obtained from LEW donors and ex vivo irradiated from 137Cs source before orthotopic SITx. Donor BM was obtained by flushing the tibias and femurs, and a total of 2.5 × 108 cells was intravenously infused into recipients on the day of SITx. FK 506 (1 mg/kg/d) was intramuscularly given to
Results
All recipients of unmodified intestinal grafts died of GVHD despite simultaneous donor BM infusion in some cases. Median survivals were 63 and 51 days with and without simultaneous donor BM infusion, respectively (n = 5 for each). Graft irradiation (10 Gy) effectively prevented GVHD, and median animal survival was prolonged to 92.5 days without BM infusion. Further prolongation was achieved by adjunct BM. All animals in this group survived for more than 100 days (n = 6). Percentages of donor
Discussion
Survival of graft passenger leukocytes was proved when donor leukocytes (microchimerism) were found in recipients whose allografts had been functioning for decades.1, 2 The implication was that donor hematopoietic progenitor cells, including prulipotent stem cells, present in the transplanted organ had migrated and survived in the recipient. Before this finding, transplantation had been defined largely in terms of a unidirectional immune reaction, and this one-way paradigm had failed to
References (4)
- et al.
Lancet
(1992) - Starzl TE, Zinkernagel R: N Engl J Med 339:24,...
Cited by (9)
Pathophysiology of intestinal grafts
2012, Bulletin de l'Academie Nationale de MedecineAbrogation of the alloreactive responses of cadaveric donor intestinal lymphocytes by intraoperative campath-1H exposure
2005, Transplantation ProceedingsPost-Transplant Management
2009, Pediatric Solid Organ Transplantation