Elsevier

Transplantation Proceedings

Volume 31, Issues 1–2, February–March 1999, Pages 565-566
Transplantation Proceedings

Proceedings of the XVIIth World Congress of the Transplantation Society
Immunomodulation of intestinal transplant with allograft irradiation and simultaneous donor bone marrow infusion

https://doi.org/10.1016/S0041-1345(98)01556-5Get rights and content

Section snippets

Methods

Inbred male Lewis (LEW, RT11) and Brown Norway (BN, RT1n) rats were purchased from Harlan Sprague Dawley, Inc., (Indianapolis, IN) and used as donors and recipients, respectively. Small intestinal grafts were obtained from LEW donors and ex vivo irradiated from 137Cs source before orthotopic SITx. Donor BM was obtained by flushing the tibias and femurs, and a total of 2.5 × 108 cells was intravenously infused into recipients on the day of SITx. FK 506 (1 mg/kg/d) was intramuscularly given to

Results

All recipients of unmodified intestinal grafts died of GVHD despite simultaneous donor BM infusion in some cases. Median survivals were 63 and 51 days with and without simultaneous donor BM infusion, respectively (n = 5 for each). Graft irradiation (10 Gy) effectively prevented GVHD, and median animal survival was prolonged to 92.5 days without BM infusion. Further prolongation was achieved by adjunct BM. All animals in this group survived for more than 100 days (n = 6). Percentages of donor

Discussion

Survival of graft passenger leukocytes was proved when donor leukocytes (microchimerism) were found in recipients whose allografts had been functioning for decades.1, 2 The implication was that donor hematopoietic progenitor cells, including prulipotent stem cells, present in the transplanted organ had migrated and survived in the recipient. Before this finding, transplantation had been defined largely in terms of a unidirectional immune reaction, and this one-way paradigm had failed to

References (4)

  • T.E. Starzl et al.

    Lancet

    (1992)
  • Starzl TE, Zinkernagel R: N Engl J Med 339:24,...
There are more references available in the full text version of this article.

Cited by (9)

  • Pathophysiology of intestinal grafts

    2012, Bulletin de l'Academie Nationale de Medecine
  • Post-Transplant Management

    2009, Pediatric Solid Organ Transplantation
View all citing articles on Scopus
View full text