Immunohistochemical observations of lysozyme in the Paneth cells of specific-pathogen-free and germ-free mice

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Summary

The localization of lysozyme, which may function as an antibacterial agent, was immunohistochemically studied on the mouse Paneth cell secretory granules showing bipartite substructures (central core and peripheral halo).The lysozyme immunoreactivity was observed in the core, but not in the halo.Even in germ-free mice, Paneth cells have many secretory granules and their cores show lysozyme immunoreactivity.It seems likely that mice Paneth cells possess the ability to produce secretory granules containing lysozyme in disregard of the intestinal bacterial milieu.

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      2019, Developmental and Comparative Immunology
      Citation Excerpt :

      The present study demonstrated that GIIA sPLA2-ex cells in the suncus intestine are found not only in the crypt but also in the villus. Interestingly, in a previous study of suncus conducted by us, the distribution of lysozyme-ip cells, which are AMPs produced in the Paneth cells of humans and rodents, were found to be similar to that of GIIA sPLA2-ex cells observed in the current study (Klockars and Osserman, 1974; Klockars and Reitamo, 1975; Satoh et al., 1988; Takemi et al., 2019). Paneth cells are reportedly absent in the suncus intestine (Kurohmaru et al., 1980), suggesting that suncus may have a novel type of intestinal epithelial cells that are specialized for producing AMPs.

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    Authors' address: Dr.Yohichi Satoh, Department of Anatomy, Asahikawa Medical College, Nishikagura 4-5, Asahikawa 078, Japan.

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