Angiotensin II interacts with nitric oxide-cyclic GMP pathway in the central control of drinking behaviour: mapping with c-fos and NADPH-diaphorase
Section snippets
Animals
Male rats (Lister hooded, Olac, Bicester, U.K.), average body weight 250–280 g, were housed singly (cages 38×25×18 cm) under reversed light-dark cycles (lights off 09.00, 12 h each phase) in a temperature-controlled room. Food (SDS commercial pellets) and water were available ad libitum.
Cannulation
Lateral ventricular cannulation: All rats were anaesthetized with avertin (tribromoethanol: tertiary amyl alcohol, 1 ml/100 g body weight; Fluka AG, Germany). The rat's head was fixed in a stereotaxic frame and a
Experiment 1: The inhibitory effects of L-NAME on water ingestion and the counter effects of l-arginine
The amounts of water intake in the first 15 min after the injections were significantly different among four groups (one-way ANOVA; F=31.85, P<0.001, Fig. 1). Rats (n=8) infused with i.c.v. saline followed by 25 pmol angiotensin II drank 8.0±1.84 ml in the first 15 min, but those infused with either 250 μg (n=11) or 125 μg L-NAME (n=7) and then angiotensin II drank only 2.8±1.08 or 4.4±1.19 ml. The effects of L-NAME were blocked by co-injection of 500 μg l-arginine rats. Rats (n=8) infused with i.c.v. l
Nitric oxide-cGMP pathway involvement in angiotensin II-induced drinking behaviour
Central actions of angiotensin II have been extensively studied in the past two decades since Epstein et al. discovered the angiotensin II elicited drinking when centrally administrated.[10]A brain renin-angiotensin system is now known to exist.27, 41, 42, 55Angiotensin II has many of the properties of a neurotransmitter. Neuropharmacological studies have demonstrated that angiotensin II interacts with catecholaminergic, cholinergic, and other peptidergic brain pathways in the central control
Conclusion
The results described in this paper provide behavioural and morphological evidence for angiotensin II interactions with nitric oxide in the central control of drinking behaviour, but not in the induction of c-Fos. Together with our previous finding that dizocilpine (maleate) suppressed the dipsogenic action and c-fos expression induced by angiotensin II, we postulate that the nitric oxide-cGMP pathway is involved in angiotensin II-induced behavioural responses, presumably through activation of
Acknowledgements
We thank Julie Lane, Margaret Allen, Peter Phillips, Eduado Torres and Helen Shiers for technical help, and the Medical Research Council and Cambridge Overseas Trust for support. B.Z. was in receipt of an Overseas Research Studentship.
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Central interaction between nitric oxide, lactate and glial cells to modulate water and sodium intake in rats
2022, Brain Research BulletinCitation Excerpt :Despite this, our results showed that previous inhibition of glial cell activity with FCt reduced water and sodium intake induced by L-NAME, suggesting a central interaction between NO and glial cells to modulate drinking behavior. In addition, the nitrergic system is known to participate in neuroendocrine, fluid intake, and pressor responses induced by central ANG-II (Calapai and Caputi, 1996; Zhu and Herbert, 1997; Saad et al., 1999; Reis et al., 2010). Recently, we showed that glial cells are also involved in this integrative response induced by central ANG-II (Flôr et al., 2018).
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2004, Brain ResearchCitation Excerpt :Two neuromodulator candidates are nitric oxide (NO) and angiotensin II (AngII). Nitric oxide acts as a stimulatory agent to dehydration-induced drinking, hypovolemia, salt intake, and AngII activity [18,27,49]. Evidence that the neurons involved in the central control of body fluid homeostasis can use NO as a major neuromodulator has been reported [7].