Gastrointestinal Inflammation: Inflammatory bowel disease in knockout mice

doi:10.1016/S0960-9822(00)00060-9

Current Biology

Volume 4, Issue 3, 1 March 1994, Pages 261-263

https://doi.org/10.1016/S0960-9822(00)00060-9 Get rights and content

Abstract

The development of gastrointestinal inflammation in recently generated gene-knockout mice suggests that inflammatory bowel disease in man may be caused by an inappropriate immune response to normal bacteria.

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Immunologists spend a lot of time trying to understand the the pathogenesis of immune-mediated diseases of various organ systems. Knowledge is most advanced where there are good animal models, such as spontaneous diabetes in non-obese diabetic mice, the systemic lupus erythematosis-like disease in NZB mice, arthritis after immunization of rodents with mycobacteria or collagen, and the multiple sclerosis-like lesions seen in the central nervous system of certain strains of mice and rats after

Thomas T. MacDonald, Department of Paediatric Gastroenterology, The Medical College of St Bartholomews Hospital, London EC1A 7BE, UK.

References (17)

JL Madara et al.
Characterization of spontaneous colitis in cotton top tamarins (Sanguinis oedipus) and its response to sulfasalazine
Gastroenterology
(1985)
P Mombaerts et al.
Spontaneous development of inflammatory bowel disease in T-cell receptor mutant mice
Cell
(1993)
B Sadlack et al.
Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene
Cell
(1993)
R Kühn et al.
Interleukin-10-deficient mice develop chronic enterocolitis
Cell
(1993)
K Kett et al.
Mucosal subclass distribution of immunoglobulin G-producing cells is different in ulcerative colitis and Crohn's disease of the colon
Gastroenterology
(1987)
K Fukushima et al.
Immunohistochemical characterization, distribution, and ultrastructure of lymphocytes bearing T-cell receptor γδ in inflammatory bowel disease
Gastroenterology
(1991)
TS Halstensen et al.
Epithelial deposition of immunoglobulin G1 and activated complement (C3b and terminal complement complex) in ulcerative colitis
Gastroenterology
(1990)
RE Hammer et al.
Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human β₂m: An animal model of HLA-B27-associated human disorders
Cell
(1990)

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Embryonic stem cells ameliorate piroxicam-induced colitis in IL10-/- KO mice
2007, Biochemical and Biophysical Research Communications
The primary objective of this work is to determine the repairing potential of murine embryonic stem cells (ES) in murine model of Crohn’s disease (CD). Colitis, induced in IL10−/− KO mice using piroxicam, was associated with the increased levels of IL-12. Enhanced yellow fluorescent protein (EYFP) marked murine ES cells (R1/129) and control non-fluorescent ES cells were subjected to in vitro differentiation into intestinal epithelial cells. IL 10−/− KO mice were injected with pre-differentiated ES-YFP cells and sacrificed after 2 and 3 months. Histopathological analysis of intestines demonstrated a progressive improvement in colitis (from grade-4 to grade-1 and -0) and decreased levels of IL-12 cytokine following transplantation. Fluorescent and confocal microscopy demonstrated presence of ES-EYFP cells in the colon, small intestine, liver, and thymus tissues but none in the spleen and bone marrow. The EYFP signal was not detected in sham (non-transplanted mice with induced colitis) and control IL10−/− KO mice. Engraftment, detected at 3 months post-transplant, correlated with markedly improved grading in colon histology (grade-1 or -0) and weight gain, as well as with decreased rectal prolapses. In vitro pre-differentiated ES cells migrated and homed exclusively into the colon, small intestine, and the liver, engrafted for long term, reduced inflammation and tissue damage, and restored immune balance. These findings suggest that pre-differentiated ES cells may become alternative source of stem cell therapy for CD with dual functions i.e. regenerating damaged epithelium and restoring immune imbalance occurring in this disease.
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Thomas T. MacDonald, Department of Paediatric Gastroenterology, The Medical College of St Bartholomews Hospital, London EC1A 7BE, UK.

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Abstract

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References (17)

Characterization of spontaneous colitis in cotton top tamarins (Sanguinis oedipus) and its response to sulfasalazine

Gastroenterology

Spontaneous development of inflammatory bowel disease in T-cell receptor mutant mice

Cell

Ulcerative colitis-like disease in mice with a disrupted interleukin-2 gene

Cell

Interleukin-10-deficient mice develop chronic enterocolitis

Cell

Mucosal subclass distribution of immunoglobulin G-producing cells is different in ulcerative colitis and Crohn's disease of the colon

Gastroenterology

Immunohistochemical characterization, distribution, and ultrastructure of lymphocytes bearing T-cell receptor γδ in inflammatory bowel disease

Gastroenterology

Epithelial deposition of immunoglobulin G1 and activated complement (C3b and terminal complement complex) in ulcerative colitis

Gastroenterology

Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human β₂m: An animal model of HLA-B27-associated human disorders

Cell

Cited by (45)

Embryonic stem cells ameliorate piroxicam-induced colitis in IL10-/- KO mice

Development and function of organized gut-associated lymphoid tissues

Role of goblet cells in intestinal barrier and mucosal immunity

Malvidin 3-Glucoside Modulated Gut Microbial Dysbiosis and Global Metabolome Disrupted in a Murine Colitis Model Induced by Dextran Sulfate Sodium

In Vivo Validation Model of a Novel Anti-Inflammatory Scaffold in Interleukin-10 Knockout Mouse

The proton-activated receptor GPR4 modulates intestinal inflammation

Current Biology

DispatchGastrointestinal Inflammation: Inflammatory bowel disease in knockout mice

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Gastroenterology

Cell

Cell

Cell

Gastroenterology

Gastroenterology

Gastroenterology

Cell

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Gastrointestinal Inflammation: Inflammatory bowel disease in knockout mice