Abstract
Colonic function in rats was investigated up to14 days following exposure to whole-body gammairradiation (8 Gy) using a combination of in vivo and invitro approaches. Water and electrolyte fluxes were measured in vivo under anesthesia by insertionof an agarose cylinder into the descending colon.Short-circuit current responses (Isc; basal,agonist-stimulated) of distal colon were measured invitro as were mannitol and sodium fluxes. Water andelectrolyte absorption (Na, Cl) was markedly reduced atfour days after irradiation but returned to normal atseven days. Potassium secretion was increased from one to seven days after exposure. There were nodifferences in basal Isc, Na, or mannitolfluxes at four days but responses to secretagogues(5-hydroxytryptamine, forskolin, carbachol) wereattenuated. No morphological alterations were associatedwith these functional modifications.
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REFERENCES
Curran PF, Schwartz GF: Na, Cl and water transport by rat colon. J Gen Physiol 43:555-571, 1960
Edmonds CJ: Transport of sodium and secretion of potassium and bicarbonate by the colon of normal and sodium-depleted rats. J Physiol 193:589-602, 1967
Devroede GJ, Phillips SF: Conservation of sodium, chloride and water by the human colon. Gastroenterology 56:101-109, 1969
Rajendran VM, Binder HJ: Ion transport in rat colon. Adv Comp Environ Physiol 16:113-137, 1993
Fordtran JS, Rector FC, Carter NW: Theme chanisms of sodium absorption in the human small intestine. J Clin Invest 47:884-900, 1968
Pedley KC, Naftalin RJ: Evidence from fluore scence microscopy and comparative studies that rat, ovine and bovine colonic crypts are absorptive. J Physiol 460:525-547, 1993
Singh SK, Binder HJ, Boron WF, Geibel JP: Fluid absorption in isolated perfused colonic crypts. J Clin Invest 96:2373-2379, 1995
Conard RA: Some effects of ionizing radiation on the physiology of the gastrointestinal tract: A review. Radiat Res 5:167- 188, 1956
Geraci P, Jackson KL, Mariano MS: Protection against the derangements associated with acute intestinal death. Pharmacol Ther 39:45-57, 1988
Gunter-Smith PJ: Effect of ionizing radiation on gastrointestinal physiology. InMilitary Radiobiology. JJ Conklin, PL Walker (eds). Orlando, Academic Press, 1987, pp 135-152
Quastler H: The nature of intestinal radiation death. Radiat Res 4:303-320, 1956
Potten CS: A comprehensive study of the radiobiological response of the murine (BDF1) intestine. Int J Radiat Biol 58:925-973, 1990
Gunter-Smith PJ: Gamma radiation affects active electrolyte transport by rabbit ileum: Basal Na and Cl transport. Am J Physiol 250:G540-G545, 1986
Gunter-Smith PJ: Gamma radiation affects active electrolyte transport by rabbit ileum. II. Correlation of alanine and theophylline response with morphology. Radiat Res 117:419-432, 1989
MacNaughton WK, Leach KE, Prud'homme-Lalonde L, Ho W, Sharkey KA: Ionizing radiation reduces neurally evoked electrolyte transport in rat ileum through a mast-cell-dependent mechanism. Gastroenterology 106:324-335, 1994
Debongnie JC, Philipps SF: Capacity of the human colon to absorb fluid. Gastroenterology 74:698-703, 1978
Zammit PS, Mendizabal M, Naftalin RJ: Effects on fluid and Na+ flux of varying luminal hydraulic resistance in rat colon in vivo. J Physiol 477:539-548, 1994
Lebrun F, Francois A, Vergnet M, Aigueperse J, Gourmelon P & Griffiths NM: (Abstract) Modification of rat intestinal muscarinic cholinergic receptors by ionizing radiation. Proceedings of the International Congress on Radiation Protection, Vienna, April, P24-21, 1996
Becciolini A: Relative radiosensitivities of the small and large intestine. Adv Radiat Biol 12:83-128, 1987
Sunter JP, Watson AJ, Wright NA, Appleton DR: Cell proliferation at different sites along the length of the rat colon. Virchows Arch B Cell Pathol 32:75-87, 1979
Hagemann RF, Lesher S: Irradiation of the G.I. tract: compensatory responses of stomach, jejunum and colon. Br J Radiol 44:599-602, 1971
Empey LR, Papp JD, Jewell LD, Fedorak RN: Mucosal protective effects of vitamin E and misoprostol during acute radiation-induced enteritis in rats. Dig Dis Sci 37:205-214, 1992
MacNaughton WK, Leach KE: Ionizing radiation-induced intestinal hypersecretion is region-specific and 5-HT3-dependent. Gastroenterology 104(suppl):A303, 1995
Sullivan MF: Sodium, water and B12 absorption in irradiated rats: Influence of bile. InGastrointestinal Radiation Injury. MF Sullivan (ed). New York, Excerpta Medica, 1966, pp 216-226
Walker RI, Porvaznik M: Association of leukopenia and intestinal permeability with radiation-induced sensitivity to endotoxin. Life Sci 23:2315-2322, 1978
Porvaznik M: Tight junction disruption and recovery after sublethal γ irradiation. Radiat Res 78:233-250, 1979
Naftalin RJ, Pedley KC: The pericryptal fibroblast layer in rat descending colon sustains large transcryptal Na+ gradients. J Physiol 482P:3P, 1995
Jackson KL, Rhodes R, Entenman C: Electrolyte excretion in the rat after severe intestinal damage by x-irradiation. Radiat Res 8:361-373, 1958
Jackson BT: Bowel damage from radiation. Proc R Soc Med 69:683-686, 1976
Harris J, Shields R: Absorption and secretion of water and electrolytes by the intact human colon in diffuse untreated proctocolitis. Gut 11:27-33, 1970
Linard C, Esposito V, Aigueperse J, Gourmelon P: Influence of irradiation on gastrin releasing peptide. In10th International Congress of Radiation Research Congress Proceedings. U Hagen, H Jung, C Streffer (eds). 1995, P15-8
Traynor TR, O'Grady SM: Regulation of colonic ion transport by GRP. I GRP stimulates transepithelial K and Na secretion. Am J Physiol 270:C848-C858, 1996
François A, Aigueperse J, Gourmelon P, MacNaughton WK, Griffiths NM: Exposure to ionizing radiation modifies neurallyevoked electrolyte transport and some inflammatory responses in rat colon in vitro. Int J Radiat Biol (in press).
Zünd G, Madara JL, Dzus AL, Awtrey CS, Colgan SP: Interleukin-4 and interleukin-13 differentially regulate epithelial chloride secretion. J Biol Chem 271:7460-7464, 1996
Summers RW, Flatt AJ, Prihoda MJ, Mitros FA: Effect of irradiation on morphology and motility of canine small intestine. Dig Dis Sci 32:1402-1410, 1987
Otterson MF, Sarna SK, Leming SC, Moulder JE, Fink JG: Effects of fractionated doses of ionizing radiation on colonic motor activity. Am J Physiol 263:G518-G526, 1992
Krantis A, Rana K, Harding RK: The effects of γ-radiation on intestinal motor activity and fecal pellet expulsion in the guinea pig. Dig Dis Sci 41:2307-2316, 1996
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Dublineau, I., Ksas, B., Aigueperse, J. et al. In Vivo Alterations of Fluid and Electrolyte Fluxes in Rat Colon by Gamma Irradiation. Dig Dis Sci 43, 652–662 (1998). https://doi.org/10.1023/A:1018839930552
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DOI: https://doi.org/10.1023/A:1018839930552