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In Vivo Alterations of Fluid and Electrolyte Fluxes in Rat Colon by Gamma Irradiation

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Abstract

Colonic function in rats was investigated up to14 days following exposure to whole-body gammairradiation (8 Gy) using a combination of in vivo and invitro approaches. Water and electrolyte fluxes were measured in vivo under anesthesia by insertionof an agarose cylinder into the descending colon.Short-circuit current responses (Isc; basal,agonist-stimulated) of distal colon were measured invitro as were mannitol and sodium fluxes. Water andelectrolyte absorption (Na, Cl) was markedly reduced atfour days after irradiation but returned to normal atseven days. Potassium secretion was increased from one to seven days after exposure. There were nodifferences in basal Isc, Na, or mannitolfluxes at four days but responses to secretagogues(5-hydroxytryptamine, forskolin, carbachol) wereattenuated. No morphological alterations were associatedwith these functional modifications.

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Dublineau, I., Ksas, B., Aigueperse, J. et al. In Vivo Alterations of Fluid and Electrolyte Fluxes in Rat Colon by Gamma Irradiation. Dig Dis Sci 43, 652–662 (1998). https://doi.org/10.1023/A:1018839930552

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