Abstract
Clinical, pathological, and virological analysisincluding hypervariable region-1 of hepatitis C virus(HCV) was performed to predict the effect of interferon(IFN) on 41 patients with chronic hepatitis type C. The low virus load, low frequency ofthe mutation in the hypervariable region-1 as the changeof amino acid and high level of serum aminotransferasemake one estimate the good effect of IFN on patients with HCV. Mutation in the hypervariableregion-1 of HCV measured by fast assay fluorescencesingle-stranded conformational polymorphism was morefrequent in nonresponders to IFN than responders. Themost frequently mutated position was amino acidnumber 406. This indicates that the specific mutationsite might affect the response of IFN.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Preston H, Wright TL: Interferon therapy for hepatitis C. Lancet 348:973–974, 1996
Davis GL: Prediction of response to interferon treatment of chronic hepatitis C. J Hepatology 21:1–3, 1994
Okada S, Akahane Y, Suzuki H, Okamoto H, Mishiro S: The degree of variability in the amino terminal region of the E2/NS1 protein of hepatitis C virus correlates with responsiveness to interferon therapy in viremic patients. Hepatology 126:619–624, 1992
Enomoto H, Sakuma I, Asahina Y, Kurosakio M, Murakami T, Yamamoto C, Ogura Y, Izumi N, Marumo F, Sato C: Mutations in the nonstructural protein 5A gene and response to interferon in patients with chronic hepatitis C virus 1b infection. N Engl J Med 334:77–81, 1996
De Groote J, Desmet VJ, Gedigk P, Korb G, Popper H, Poulsen H, Scheuer PJ, Schmid M, Thaler H, Uehlinger E: A classification of chronic hepatitis. Lancet 2:626–628, 1968
Knodell RG, Ishak KG, Black WC, Chen TS, Craig R, Kaplowitz N, Kiernan TW, Wollman J: Formulation and application of numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis. Hepatology 1:431–435, 1981
Ichida F, Tsuji, T, Omata M, Ichida T, Inoue K, Yamada G, Hino K, Yokosuka O, Suzuki H: New Inuyama classification: New criteria for histological assessment of chronic hepatitis. Int Hepatol Commun 6:112–119, 1996
Chomczynski P, Sacchi N: Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159, 1987
Sambrook J, Fritsch EF, Maniatis T (eds): Cold Spring Harbor, New York, Cold Spring Harbor Laboratory, Molecular Cloning 2, 2nd ed. 1989, pp 20–21
Schulhof JC, Molko D, Teoule R: The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups. Nucl Acids Res 15:397–416, 1987
Hultman T, Stahl S, Hornes E, Uhlen M: Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res 17:4937–4946, 1989
Sekiya H, Kato N, Ootsuka Y, Nakazawa T, Yamauchi K, Shimotohno K: Genetic alterations of the putative envelope proteins encoding region of the hepatitis C virus in the progression to relapsed phase from acute hepatitis: Humoral immune response to hypervariable region 1. Int J Cancer 57:664–670, 1994
Sherman KE, Andreatta-C, Obrien-J, Gutierrez A, Harris R: hepatitis C in human immunodeficiency virus-coinfected patients: Increased variability in the hypervariable envelope coding domain. Hepatology 23:688–694, 1996
Weiner AJ, Geyson HM, Christopherson C, Hall JE, Mason TJ, Saracco G, Bonino F, Crawford K, Marion CD, Crawford KA: Immune selection of hepatitis C virus (HCV) putative envelope glycoprotein variants: potential role in chronic HCV infections. Proc Natl Acad Sci USA 89:3468–3472, 1992
Kumar U, Nonjardino J, Thomas HC: Hypervariable region of hepatitis C virus envelope glycoprotein (E2/NS1) in an agammaglobulinemic patient. Gastroenterology 106:1072–1075, 1994
Zibert A, Schreier E, Roggendorf M: Antibodies in human sera specific to hypervariable region 1 of hepatitis C virus can block viral attachment. Virology 208:653–661, 1995
Shindo M, Hamada K, Koya S, Arai K, Sokawa Y, Okuno T: The clinical significance of changes in genetic heterogeneity of the hypervariable region 1 in chronic hepatitis C with interferon therapy. Hepatology 24:1018–1023, 1996
Rights and permissions
About this article
Cite this article
Yamada, T., Hashimoto, Y., Saitoh, S. et al. Prediction of Effect of Interferon on Chronic Hepatitis C. Dig Dis Sci 42, 2270–2276 (1997). https://doi.org/10.1023/A:1018866717753
Issue Date:
DOI: https://doi.org/10.1023/A:1018866717753