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Enhanced Intestinal Expression of Heat Shock Protein 70 in Patients with Inflammatory Bowel Diseases

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Abstract

Heat shock protein (Hsp) 70 is stress-inducibleand exhibits both protective and antigenic properties.This study investigated the mucosal expression of theconstitutive (Hsp70c) and inducible form (Hsp70i) as well as antibodies against human Hsp70 ininflammatory bowel disease and controls. Biopsies wereassessed by immunoblot and immunofluorescence, resectionspecimens by immunohistochemistry, and mucosal antibody content by isoelectric focusing.Compared to controls, expression of Hsp70 was enhancedin ulcerative colitis (P < 0.05), less so in Crohn'sdisease and infectious colitis. Strong epithelial staining was found for Hsp70c and Hsp70i inboth diseases. Mucosal and submucosal mononuclear cellsshowed enhanced Hsp70c expression in Crohn's disease andto a lesser degree in ulcerative colitis. Antibodies of isotypes A or M were detected in nearly allpatients and controls. The different pattern of Hsp70expression in Crohn's disease compared to ulcerativecolitis points to a distinct protective andimmunological function, whereas a role in autoimmunity seemsunlikely.

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Authors
  1. Diether Ludwig
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  2. Maren Stahl
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  3. M. El Taher Ibrahim
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  4. Bjorn E. Wenzel
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  5. Dorota Drabicki
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  6. Anette Wecke
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  7. Klaus Fellermann
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  8. Eduard F. Stange
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Ludwig, D., Stahl, M., Ibrahim, M.E.T. et al. Enhanced Intestinal Expression of Heat Shock Protein 70 in Patients with Inflammatory Bowel Diseases. Dig Dis Sci 44, 1440–1447 (1999). https://doi.org/10.1023/A:1026616221950

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