Abstract
To determine the clinical significance ofHelicobacter pylori seropositivity and seronegativity inhealthy blood donors, we carried out a serologicalevaluation of Helicobacter pylori status and endoscopy in a healthy blood donors population. In all,1010 donors were screened for Helicobacter pylori by IgGELISA and assessed for pepsinogen I and gastrin levelsby RIA; 298 IgG seropositive and 61 seronegative subjects underwent endoscopy with biopsies. Of359, 165 were also tested for CagA by western blotting.Of the 298 IgG seropositives, 274 were shown to beinfected on biopsy testing. Endoscopy revealed 70 peptic ulcers, 41 cases of erosive duodenitis,and two gastric cancers. In all 105 seropositive donorswere tested for CagA and 69 were CagA positive [34/58gastritis (58.6%), 24/35 duodenal ulcer (68.6%) and 11/12 gastric ulcer (91.6%)].Histologically active/chronic gastritis was associatedwith CagA: 88.4% vs 50% (CagA seropositive vsseronegative). Of the 61 IgG seronegatives, 59 werenegative on biopsy testing. At endoscopy three had duodenitis. Ofthe 60/61 IgG seronegatives tested for CagA, one had amoderate reaction. Duodenal ulcer donors showed higherpepsinogen I levels than donors without duodenal ulcers (97.7 μg/ml vs 80.9 μg/mlrespectively). Screening for Helicobacter pylori andanti-CagA seropositivity and pepsinogen I can identifyindividuals likely to have gastroduodenal pathology evenin the absence of symptoms.
Similar content being viewed by others
REFERENCES
Vaira D, Holton J, Miglioli M, e t al: Peptic ulcer disease and Helicobacter pyloriinfection. Curr Opin Gastroenterol 10:98–104, 1994
Blaser MJ, Chyou PH, Nomura A: Age at establishment of He licobacte r pylori infection and gastric carcinoma, gastric ulcer and duodenal ulcer risk. Cancer Res 55:562–565, 1995
Hu PJ, Li YY, Zhou MH, Chen MH, Du GG, Huang BJ, Mitchell HM, Hazell SL: Helicobacter pyloriassociated with high prevalence of duodenal ulcer disease and low prevalence of gastric cance r in a deve loping nation. Gut 36:198–202, 1995
The Eurogast Study Group: An international association between Helicobacter pylori infection and gastric cancer. Lancet 1:1359–1362, 1993
Sitas F, Forman D, Yarnell JWG, et al: Helicobacter pyloriinfection rates in relation to age and social class in a population of Welshmen. Gut 32:25–28, 1991
Graham DY, Malaty HM, Evans DG, et al: Epidemiology of Helicobacter pyloriin an asymptomatic population in the United States. Gastroenterology 100:1495–1501, 1991
Pate l P, Khulusi S, Mendall MA, et al: Prospe ctive scree ning of dyspeptic patients by Helicobacter pylorise rology. Lance t 346:1315–1318, 1995
Schlemper RJ, van der Werf SDJ, Vande rbroucke JP, et al: Seroepidemiology of gastritis in Japanese and Dutch working populations: Evidence for the development of atrophic gastritis that is not related to Helicobacter pylori.Gut 37:199–204, 1995
Mossi S, Meyer-Wyss Beat, Renner EL, et al: Infl uence of Helicobacter pylori,sex, and age on serum gastrin and pepsinogen concentrations in subjects without symptoms and patients with duodenal ulcers. Gut 34:752–756, 1993
Figura N, Bugnoli M, Cusi MG, e t al: Pathogenic mechanisms of Helicobacter pylori: Production of cytotoxin. In: Helicobacter pylori,Gastritis and Peptic Ulcer. Be rlin, Springer-Ve rlag, 1990, pp 86–95
Cover TL, Dooley CP, Blase r MJ: Characte rizationn of and human se rological re sponse to proteins in Helicobacter pyloribroth culture supernatants with vacuolising cytotoxin activity. Infect Immun 58:603–610, 1990
Crabtree JE, Taylor JD, Wyatt JI, et al: Mucosal IgA recognition of Helicobacter pylori120 kDa protein, peptic ulceration and gastric pathology. Lance t 338:332–335, 1991
Figura N: Progress in defining the inflammatory cascade. Eur J Gastroente rol Hepatol 7:296–302, 1995
Vaira D, Miglioli M, Mulè P, et al: Prevalence of peptic ulcer in Helicobacter pyloripositive blood donors. Gut 35:309–312, 1994
Office of Population Censuses and Surveys: Standard Occupational Classification. Government Statistical Service, London, HMSO, 1991
Biasco G, Pagane lli GM, D Vaira, et al: Serum pepsinogen I and II concentrations and IgG antibody to Helicobacter pyloriin dyspeptic patients. J Clin Pathol 46:826–828, 1993
Blakstone MO: Endoscopic Interpretation. New York, Raven Press, 1984
Genta RM, Dixon F: The Sydney system revised. The Houston international gastritis workshop. Am J Gastroenterol 90:1039–1041, 1995
Talley NJ, Zinsme ister AR, Schleck CD, e t al: Dyspepsia and dyspepsia subgroups: A population-based study. Gastroente rology 102:1259–1268, 1992
Dooley CP, Cohen H, Fitzgibbons PL, et al: Prevalence of Helicobacter pyloriinfection and histologic gastritis in asymptomatic persons. N Engl J Med 321:1562–1566, 1989
Buckley M, O'shena J, English L, O'Morain C: Helicobacter pyloricauses asymptomatic gastroduodenal pathology. Am J Gastroente rol 89:1302 (A70), 1994
Sipponen P, Varis K, Frankl O, Korri U-M, Seppa K, Siurala M: Cumulative ten ye ar risk of symptomatic duodenal and gastric ulcer in patients with or without chronic gastritis. Scand J Gastroenterol 25:966–973, 1990
Pretolani S, Figura N, Miglio F, e t al: Gastroduodenal endoscopic lesions in the population of San Marino: Association with Helicobacter pyloriand anti-CagA serum antibodies. Ital J Gastroente rol 27( suppl 1):211, 1995
Anand BS, Malaty H, Genta RM, e t al: Peptic ulcer is rare in asymptomatic adults with Helicobacter pyloriinfection. Gut 37( suppl 1):A38, 1995
Crabtree JE, Farmery SM, Lindley JD, et al: CagA/cytotoxic strins of Helicobacter pyloriand interleukine-8 in gastric epithelial cells. J Clin Pathol 47:945–950, 1994
Cover T, Glupczynski Y, Lage A, e t al: Serologic detection of infection with CagA+ Helicobacter pyloristrains. J Clin Microbiol 33:1496–1500, 1995
Czinn SJ, Be rtram TA, Murray PD, et al: Relationship between gastric inflammatory response and symptoms in patients infected with Helicobacter pylori.Scand J Gastroenterol 26(suppl 181):33–37, 1991
Figura N, Guglie lme tti P, Rossolini A, et al: Doe s the production of cytotoxin by Campylobacter pyloriplay a pathological role in the development of peptic ulcer? In:Gastroduodenal Pathology and Campylobacter pylori.F Megraud, H Lamouliatte (eds). Amsterdam, Elsevier, 1989, pp 385–389
Husson MO, Gottrand F, Vacheea A, et al: Importance in diagnosis of gastritis of detection by PCR of the CagA gene in Helicobacter pyloristrains isolated from children. J Clin Microbiol 33:3300–3303, 1995
Rights and permissions
About this article
Cite this article
Menegatti, M., Holton, J., Figura, N. et al. Clinical Significance of Helicobacter pylori Seropositivity and Seronegativity in Asymptomatic Blood Donors. Dig Dis Sci 43, 2542–2548 (1998). https://doi.org/10.1023/A:1026663022278
Issue Date:
DOI: https://doi.org/10.1023/A:1026663022278