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Donor origin of the in vitro haematopoietic microenvironment after marrow transplantation in man

Nature volume 298, pages 280–283 (1982)Cite this article

Abstract

The method for long-term culture of marrow cells in vitro as described by Dexter1 has recently been successfully applied to human marrow2,3 and is dependent on the development of an adherent stromal cell layer consisting of cells described as “endothelial-like cells, fat cells, and macrophages” 4. The present study was designed to determine the origin and composition of the stromal cells forming the in vitro ‘microenvironment’ and maintaining haematopoeisis in long-term cultures grown from marrows of 14 patients who received marrow transplants from HLA identical siblings of the opposite sex. The presence of a Y chromosome was used as a marker to establish the donor or recipient origin of the cells. We found that the stromal cells became progressively donor in origin with time after transplantation and some reacted with antibody directed against factor VIII-associated antigen. In addition, donor-derived in vitro stromal cells synthesized both interstitial and basal lamina collagen types, indicating that the in vitro microenvironment is transplantable and composed in part of endothelial-like cells.

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Authors and Affiliations

  1. The Medical Service, Veterans Administration Medical Center Division of Oncology, 4435 Beacon Avenue South, Seattle, Washington, 98108, USA

    Armand Keating & Jack W. Singer

  2. Divisions of Oncology and Medical Genetics, Department of Medicine and Department of Pathology, University of Washington, Seattle, Washington, 98195, USA

    Paul D. Killen, Gary E. Striker, Armi C. Salo, David Thorning & Philip J. Fialkow

  3. Fred Hutchinson Cancer Research Center, Seattle, Washington, 98104, USA

    Jean Sanders & E. Donnall Thomas

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  1. Armand Keating
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  9. Philip J. Fialkow
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Keating, A., Singer, J., Killen, P. et al. Donor origin of the in vitro haematopoietic microenvironment after marrow transplantation in man. Nature 298, 280–283 (1982). https://doi.org/10.1038/298280a0

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