Abstract
The homologue of the viral Kirsten ras (ν-Ki-ras) gene found in the human lung carcinoma cell line, Calu-1, has an intron–exon structure similar to that of the human homologue of the viral Harvey ras (v-Ha-ras) gene. A second, potential fourth coding exon is present in the human Ki-ras gene and similar sequences are found in the Kirsten murine sarcoma virus. Cysteine is encoded at the twelfth amino acid position, suggesting that the Calu-1 Ki-ras gene has undergone a mutational activation at the same position as the human Ha-ras gene of the bladder carcinoma cell line, T24. A comparison of their predicted amino acid sequences suggests that ras proteins have a ‘constant’ region and a ‘variable’ region. Here we propose a common modular structure for ras gene products in which the variable region forms a physiologically important combining site.
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Shimizu, K., Birnbaum, D., Ruley, M. et al. Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1. Nature 304, 497–500 (1983). https://doi.org/10.1038/304497a0
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DOI: https://doi.org/10.1038/304497a0
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