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Cotransfection of ICAM-1 and HLA-DR reconstitutes human antigen-presenting cell function in mouse L cells

Abstract

THE initiation of a specific immune response is believed to require not only activation through antigen-specific receptors on T cells and B cells but also antigen-independent interactions between accessory molecules1. One such molecule is LFA-1, which enhances the avidity of interactions between T cells and antigen-presenting cells2–4, and is possibly involved in signal transduction across the T-cell membrane5. Intercellular adhesion molecule-1 (ICAM-1), a surface glycoprotein of relative molecular mass (Mr) 80,000–110,000, has been defined as a ligand for LFA-16,7, and has been shown to participate in the interaction between T cells and monocytes8. The determination of the precise contribution of such accessory molecules to antigen presentation, however, is complicated by the need to analyse against a background of multiple molecular interactions. We have investigated the role of LFA-1/ICAM-1 interactions in antigen presentation directly by quantifying the contribution of ICAM-1 expression to T-cell stimulation using L-cell transfectants that co-express ICAM-1 and HLA-DR. In the case of transfectants expressing modest levels of HLA-DR, co-expression of ICAM-1 is critical for effective HLA class H-restricted and allospecific T-cell activation, pointing to an important role for ICAM-1 in the induction of T-cell responses.

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References

  1. Springer, T. A., Dustin, M. L., Kishimoto, T. K. & Marlin, S. D. A. Rev. Immun. 5, 223–252 (1987).

    Article  CAS  Google Scholar 

  2. Gougeon, M.-L., Bismuth, G. & Theze, J. cell. Immun. 95, 75–83 (1985).

    Article  CAS  Google Scholar 

  3. Dougherty, G. J. & Hogg, N. Eur. J. Immun. 17, 943–947 (1987).

    Article  CAS  Google Scholar 

  4. Shaw, S. et al. Nature 323, 262–264 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. van Noesel, C. et al. Nature 333, 850–852 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  6. Marlin, S. & Springer, T. A. Cell 51, 813–819 (1987).

    Article  CAS  PubMed  Google Scholar 

  7. Simmons, D., Makgoba, M. W. & Seed, B. Nature 331, 624–627 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  8. Dougherty, G. J., Murdoch, S. & Hogg, N. Eur. J. Immun. 18, 35–39 (1988).

    Article  CAS  Google Scholar 

  9. Wilkinson, D. et al. J. exp. Med. 167, 1442–1458 (1988).

    Article  CAS  PubMed  Google Scholar 

  10. Young, J. A. T., Wilkinson, D., Bodmer, W. F. & Trowsdale, J. Proc. natn. Acad. Sci., U.S.A. 84, 4929–4933 (1987).

    Article  ADS  CAS  Google Scholar 

  11. Rogelj, S., Weinberg, R. A., Fanning, P. & Klagsburn, M. Nature 331, 173–175 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Rothlein, R., Dustin, M. L., Marlin, S. D. & Springer, T. A. J. Immun. 137, 1270–1274 (1986).

    CAS  PubMed  Google Scholar 

  13. Golde, W. T. et al. J. exp. Med. 161, 635–640 (1985).

    Article  CAS  PubMed  Google Scholar 

  14. Barbosa, J. A. et al. Proc. natn. Acad. Sci., U.S.A. 81, 7549–7553 (1984).

    Article  ADS  CAS  Google Scholar 

  15. Eckels, D. D., Sell, T. W., Long, E. O. & Sekaly, R. P. Hum. Immun. 21, 173–181 (1988).

    Article  CAS  PubMed  Google Scholar 

  16. Hildreth, J. E. K., Gotch, F. M., Hildreth, P. D. K. & McMichael, A. J. Eur. J. Immun. 13, 202–208 (1983).

    Article  CAS  Google Scholar 

  17. Lampson, L. A. & Levy, R. J. Immun. 125, 293–299 (1980).

    CAS  PubMed  Google Scholar 

  18. Marrack, P. & Kappler, J. Nature 332, 840–843 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  19. Matis, L. A., Soerger, S. B., McElliot, D. L., Fink, P. J. & Hedrick, S. M. Cell 51, 59–69 (1988).

    Article  Google Scholar 

  20. Collins, T. et al. Proc. natn. Acad. Sci., U.S.A. 81, 4917–4921 (1984).

    Article  ADS  CAS  Google Scholar 

  21. Dustin, M. L., Rothlein, R., Bhan, A. K., Dinarello, C. A. & Springer, T. A. J. Immun. 137, 245–255 (1986).

    CAS  PubMed  Google Scholar 

  22. Radka, S. F., Charron, D. J. & Brodsky, F. M. Hum. Immun. 16, 390–400 (1986).

    Article  CAS  PubMed  Google Scholar 

  23. Dustin, M. L., Singer, K. H., Tuck, D. T. & Springer, T. A. J. exp. Med. 167, 1323–1340 (1988).

    Article  CAS  PubMed  Google Scholar 

  24. Bottazzo, G. F., Pujol-Borrell, R., Hanafusa, T. & Feldmann, M. Lancet ii, 1115–1117 (1983).

    Article  Google Scholar 

  25. Gregory, C. D., Murray, R. J., Edwards, C. F. & Rickinson, A. B. J. exp. Med. 167, 1811–1824 (1988).

    Article  CAS  PubMed  Google Scholar 

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Altmann, D., Hogg, N., Trowsdale, J. et al. Cotransfection of ICAM-1 and HLA-DR reconstitutes human antigen-presenting cell function in mouse L cells. Nature 338, 512–514 (1989). https://doi.org/10.1038/338512a0

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