Alimentary TractEnteroendocrine localization of GLP-2 receptor expression in humans and rodents☆,☆☆
Section snippets
Animals
Mice transgenic for the rat GLP-2R complementary DNA (cDNA) under the control of the intestinal fatty acid–binding protein (FABP) promoter14 were generated and propagated in accordance with the guidelines of the Toronto General Hospital Animal Care Committee. These mice were generated to assess the effects on enterocyte biology of targeted expression of the GLP-2R to a localized intestinal epithelial compartment.14 Because FABP–GLP-2R mice express high levels of the translated rat GLP-2R in the
Results
Multiple GLP-1R RNA transcripts have been detected using Northern blotting and GLP-1R–specific cDNA probes10, 24; however, whether the GLP-2R gene also gives rise to multiple transcripts is not currently known. Because messenger RNA (mRNA) transcripts for related members of the glucagon receptor superfamily potentially cross-hybridize with GLP-2R probes, we first ascertained the specificity of Northern blotting for detection of GLP-2R mRNA transcripts. RNA isolated from rat jejunum, lung,
Discussion
Our studies show that GLP-2R expression, as assessed by a combination of Northern blotting, RT-PCR, and immunocytochemistry, is predominantly restricted to the gastrointestinal tract and brain. The observation that GLP-2R–mRNA transcripts are expressed at low levels in the gastrointestinal tract, and detectable only by sensitive techniques such as Northern blotting with polyA+ RNA, RNAse protection assays, or RT-PCR, is in keeping with previous efforts that failed to detect GLP-2 binding in the
Acknowledgements
The authors thank Dr. D. Irwin for technical assistance.
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Address correspondence to: Daniel Drucker, M.D., Toronto General Hospital, 200 Elizabeth Street, CCRW3-845, Toronto, M5G 2C4, Canada. e-mail: [email protected]; fax: (416) 978-4108.
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Supported by a Senior Scientist Award (to D.D.) from the Medical Research Council (MRC) of Canada and in part by operating grants from the MRC of Canada and by an Ontario Research and Development Challenge Fund Award. Dr. Drucker is a consultant to NPS Allelix Inc., and GLP-2 is the subject of a licensing agreement between the Toronto General Hospital, University of Toronto, and NPS Allelix Inc.