Alimentary TractPermeability of the rat small intestinal epithelium along the villus-crypt axis: Effects of glucose transport☆,☆☆
Section snippets
Operative procedures
The experiments were performed on male Sprague–Dawley rats (Alab, Stockholm, Sweden) weighing 250–450 g. The animals were kept under standardized environmental conditions (22°C, 60% humidity, artificial light from 6 AM to 6 PM) in the animal quarters for at least 7 days before the experiment. The animals were deprived of food 12 hours before the experiments with full access to water. Anesthesia was induced with pentobarbitone (60 mg/kg, intraperitoneally). A tracheal cannula was inserted to
Tissue content of 51Cr-EDTA and 14C-mannitol
In 4 experiments, the tissue content of 51Cr-EDTA and 14C-mannitol was determined in an intestinal segment after a perfusion period of 4 hours with the Krebs–Henseleit solution containing glucose. A nonperfused control segment was also taken for analysis of probe content. The amount of 51Cr-EDTA and 14C-mannitol in the tissue of the test segment corresponded to 1.4% of the total amount absorbed for both isotopes and to 0.65% and 0.85% in the control tissue, respectively. The autoradiographs
Discussion
The method used in this study gave a significant and reproducible clearance of mannitol and Cr-EDTA but did not detect a significant clearance of inulin, independent of the presence of mannitol or glucose in the perfusate. The clearance values were not erroneously affected by markers left in the intestinal lumen at the end of the perfusion period, e.g., by adhering to the mucous layer. This is shown by the autoradiographic experiments and by the observation that the ClCr-EDTA in one
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Supported by Swedish Medical Research Council (2855) and Åke Wiberg Foundation.
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Address requests for reprints to: Mats Jodal, M.D., Department of Physiology, Box 432, S- 405 30 Göteborg, Sweden. e-mail: [email protected]; fax: (46) 31-773-3512.