Basic–Alimentary TractExpansion of CD8+ T cells with regulatory function after interaction with intestinal epithelial cells*,**
Section snippets
Preparation of human intestinal epithelial cells and lamina propria mononuclear cells
Surgical specimens from patients undergoing bowel resection for cancer (at least 10 cm away from tumor) at the Mount Sinai Medical Center were used as a source of IECs. IECs were isolated by a method described previously.18 Resected surgical specimens were washed extensively with phosphate-buffered saline (PBS). The mucosa was stripped off from the submucosa, minced into small pieces, and placed in 1 mmol/L dithiothreitol (Sigma Chemical Co., St. Louis, MO) for 10 minutes at room temperature to
Intestinal epithelial cells activate different subsets of T cells that represent less than 1% of the pool of peripheral blood T cells
To further study the interaction between IECs and lymphocytes, we used CFSE to assess the proliferation of T cells in IEC:T-cell cocultures. After 5 days of coculture, proliferating T cells (CD3+ CFSE low) represented 3.5%–35% of all T cells in more than 10 independent experiments (Figure 1).
Discussion
By using in vitro IEC:T-cell cocultures, we show that only a small fraction of peripheral T cells respond to IEC. IECs express several antigen-presenting molecules, such as classical class I and II molecules, nonclassical class Ib molecules (CD1d, human leukocyte antigen E, MICA/B, FcRn), and costimulatory molecules (gp180, B7h24). As expected, we found that IECs activated different subsets of T cells. Each of them may interact with a different combination of antigen-presenting and
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Cited by (0)
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Address requests for reprints to: Matthieu Allez, M.D., Immunobiology Center, Mount Sinai School of Medicine, 1425 Madison Avenue, Room 11-20, New York, New York 10029. e-mail: [email protected]; fax: (212) 987-5593.
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Supported by National Institutes of Health grants AI 23504, AI 24671, AI 44236, a Crohn's and Colitis Foundation of America fellowship award (to M.A. and I.D.), Société Nationale Française de Gastro-Entérologie (to M.A.), Institut de recherche des maladies de l'appareil digestif (to M.A.), Laboratoire Glaxo-Wellcome (to M.A.), and the Danish medical research agency (to J.B.).