Basic-Alimentary TractCARD15/NOD2 functions as an antibacterial factor in human intestinal epithelial cells☆,☆☆,★
Section snippets
Cell culture
HT-29, Caco2, T84, Colo205, SW480, WiDr, SW48.5, LS174, and SW620 cells were obtained from the American Type Culture Collection (Manassas, VA) and used for experiments after they had reached 90% to 100% confluence. HT-29 cells were cultured in Dulbecco's modified Eagle medium with l-glutamine (Cellgro Mediatech Inc., Herndon, VA) supplemented with 10% (vol/vol) fetal calf serum (Atlanta Biologicals Inc., Norcross, GA). Caco2 cells were cultured in Dulbecco's modified Eagle medium supplemented
Expression of NOD2 messenger RNA in intestinal epithelial cell lines
Expression of NOD2 messenger RNA (mRNA) was assessed by RT-PCR in several human IEC lines (T84, HT-29, Caco2, SW480, SW620, WiDr, Colo205, SW48.5, and LS174). Although previous reports have concluded on the basis of total-tissue Northern blot analysis that CARD15/NOD2 expression was confined to monocytes in peripheral blood, CARD15/NOD2 (product size: 822 bp) mRNA was present in several independently derived colonic epithelial lines, including SW480, SW620, T84, colo205, and LS174 cells (Figure
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Address requests for reprints to: Daniel K. Podolsky, M.D., Gastrointestinal Unit, Massachusetts General Hospital, 55 Fruit Street, Boston, Massachusetts 02114. e-mail: [email protected]; fax: (617) 724-2136.
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Supported in part by the National Institutes of Health grants DK43351, DK60049, and DK56754 (to D.K.P.), and DK33506 (to B.A.M.).
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