Basic–Alimentary TractA Rat Model of Chronic Gastric Sensorimotor Dysfunction Resulting From Transient Neonatal Gastric Irritation
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Animals
Male Sprague-Dawley rats were used in all the experiments (Harlan, Indianapolis, IN). The animal protocol was approved by the Institutional Animal Care and Use Committee of the University of Texas Medical Branch.
Neonatal Gastric Irritation
Ten-day-old rat pups received 0.2 mL of 0.1% iodoacetamide (IA) in 2% sucrose daily for 6 days by oral gavages. The control group received 0.2 mL of 2% sucrose.
Implantation of Balloon and Electrodes for Behavior and Electromyographic Testing
After overnight fasting, 8-week-old rats were anesthetized with intraperitoneal ketamine (60 mg/kg) and xylazine (7 mg/kg).
Neonatal IA Treatment Does Not Cause Long-Term Changes in Body Weight
Body weight was determined on days 2, 3, 4, 5, and 6 during 0.1% IA in 2% sucrose or 2% sucrose treatment and on day 18 post-treatment. Weight gain was significantly lower in the IA-treated rats during days 3–6 of the treatment period (*P < .05, Figure 1). However, by day 18, body weights in both groups were similar.
Neonatal IA Treatment Causes Mild and Transient Superficial Mucosal Injury
Gastric histology in neonatal rats 6 days following exposure to IA or sucrose (n = 8 in each group) revealed only superficial sloughing of the mucosa in the IA group without any
Discussion
In the current study, a sulfhydryl acetylating agent, IA, was used to induce a transient mild gastric inflammation in neonatal rats. This is based on reports that 0.1% IA for 5–7 days in adult rats or mice induces mild gastritis.16, 17, 18, 19 Furthermore, IA has been shown to result in acute changes of gastric sensory and motor function.8, 20 Our study showed that treatment with 0.1% IA orally once daily for 6 days in neonates induced mild but transient damage to the surface epithelium of
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Conflicts of interest: No conflicts of interest exist, and no potential investigator conflicts of interest exist.