Original CommunicationsEffect of endothelial and adventitial injury on somatostatin receptor expression*,**,★
Section snippets
Animals
Wistar rats weighing 400 to 500 g were obtained from the Animal Care Facility at the University of British Columbia and housed in a 12-hour light/dark cycle. All animal procedures were approved by the University of British Columbia Animal Care Committee, and animals were cared for in accordance with the principles contained in The Care of Experimental Animals - A Guide for Canada, published by the Canadian Council on Animal Care (1993).
Rats were anesthetized with intravenous pento-barbital and
Immunocytochemistry
Immunocytochemical staining of normal arterial samples with antibodies to SSTR1, SSTR2, and SSTR3 failed to show any significant level of immunoreactivity. The antibody to SSTR2 occasionally showed punctate staining of the surface of endothelial cells but this was barely above background levels. However, in the balloon catheter injured and dissected arteries, or dissected arteries alone, there was a clear increase in SSTR2 immunoreactivity on the endothelial cells (Fig 1, A and B).
Discussion
Previous studies using SST analogs known to activate members of the SSTR2, 3, and 5 subgroups have been successful in limiting intimal hyperplasia after vascular injury.4, 6, 10, 15, 16 However, the precise SST receptor subtype activated after injury to blood vessels has not been determined. The results of this study demonstrate that SSTR2 and 3 are present in the normal rat iliac artery and, in response to either endothelial or endothelial and adventitial injury, SSTR2 is significantly
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Cited by (11)
Somatostatin receptors
2007, xPharm: The Comprehensive Pharmacology ReferenceTNF-α decreases expression of somatostatin, somatostatin receptors, and cortistatin in human coronary endothelial cells
2005, Journal of Surgical ResearchSomatostatin regulates intracellular signaling in human carotid endothelial cells
2004, Biochemical and Biophysical Research CommunicationsCellular localization and distribution of neurokinin-1 receptors in the rat stomach
2003, Autonomic Neuroscience: Basic and ClinicalSomatostatin and the “small-for-size” liver
2019, International Journal of Molecular Sciences
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Funded in part by the Heart and Stroke Foundation of British Columbia and Yukon and the Rogers Research Foundation.
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Reprint requests: Dr York N. Hsiang, Division of Vascular Surgery, Department of Surgery, University of British Columbia, Vancouver General Hospital, Room 3100 LSP, 910 W 10th Ave, Vancouver, BC, V5Z 4E3, Canada.
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Surgery 2000;127:577–83