Abstract
RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.
MeSH terms
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Aging / genetics*
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Animals
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Gene Silencing*
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Genes, Reporter / genetics
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Hepacivirus / genetics*
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Humans
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Liver / metabolism
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Luciferases / biosynthesis
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Luciferases / genetics
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Mice
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RNA, Double-Stranded / chemistry
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RNA, Double-Stranded / genetics
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RNA, Double-Stranded / metabolism
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RNA, Small Interfering
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RNA, Untranslated / chemistry
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RNA, Untranslated / genetics*
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RNA, Untranslated / metabolism*
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RNA, Viral / genetics
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RNA, Viral / metabolism
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Substrate Specificity
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Transgenes / genetics
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Viral Nonstructural Proteins / biosynthesis
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Viral Nonstructural Proteins / genetics
Substances
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RNA, Double-Stranded
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RNA, Small Interfering
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RNA, Untranslated
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RNA, Viral
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Recombinant Fusion Proteins
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Viral Nonstructural Proteins
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Luciferases
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NS-5 protein, hepatitis C virus