Abstract
A cDNA encoding the homolog of the human pre-B-cell colony-enhancing factor (PBEF), a cytokine-like secreted protein, was isolated from a rat cDNA library. This protein existed in both the cytoplasm and nucleus of the cells, and the amount was higher in the cytoplasm than in the nucleus of proliferating PC-12 and Swiss 3T3 cells but higher in the nucleus than in the cytoplasm of the PC-12 cells treated with nerve growth factor and the 3T3 cells grown to a confluent state. Thus, the so-called PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Blotting, Northern
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Blotting, Western
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Cell Cycle
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Cloning, Molecular
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Cytokines / chemistry*
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Cytokines / genetics*
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Cytokines / metabolism
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Cytoplasm / metabolism
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DNA, Complementary / metabolism
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Electrophoresis, Polyacrylamide Gel
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Gene Library
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Immunohistochemistry
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Mice
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Molecular Sequence Data
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Nicotinamide Phosphoribosyltransferase
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PC12 Cells
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Rats
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Sequence Homology, Amino Acid
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Tissue Distribution
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Transfection
Substances
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Cytokines
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DNA, Complementary
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Nicotinamide Phosphoribosyltransferase
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nicotinamide phosphoribosyltransferase, human