Abstract
A combination of reverse transcription and polymerase chain reaction was utilized to detect baseline endothelin-1 (ET-1) mRNA expressed in renal tubules dissected from rats. 5' and 3' primers were constructed according to human ET-1 genomic DNA. Rat ET-1 mRNA was found to be expressed only in cortical through medullary collecting ducts in addition to glomeruli. Sites for tubular synthesis of ET-1 corroborate well with major ET-1 binding sites along the nephron, indicating autocrine/paracrine role of ET-1 in the renal tubules and supporting a prevailing concept on such function of ET-1 in many differing tissues.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Base Sequence
-
Blotting, Southern
-
DNA / genetics
-
DNA / isolation & purification
-
Endothelins / genetics*
-
Kidney Cortex / physiology
-
Kidney Glomerulus / physiology
-
Kidney Medulla / physiology
-
Kidney Tubules / physiology*
-
Kidney Tubules, Collecting / physiology
-
Male
-
Molecular Sequence Data
-
Oligodeoxyribonucleotides
-
Polymerase Chain Reaction / methods
-
RNA, Messenger / analysis*
-
RNA, Messenger / genetics
-
RNA-Directed DNA Polymerase
-
Rats
-
Rats, Wistar
Substances
-
Endothelins
-
Oligodeoxyribonucleotides
-
RNA, Messenger
-
DNA
-
RNA-Directed DNA Polymerase