Abstract
Epidermal growth factor receptors and insulin like growth factor-I receptors were co-expressed on two gastric and three colorectal tumour cell lines. Previous studies have shown that gastrin receptors were also expressed at a low level or two of these cell lines. Both TGF alpha and IGF-I promoted cell growth in all of the cell lines tested. The cell doubling time of a colorectal cell line was reduced from 48 to 30-34 h. Furthermore the effects of the growth factors were additive. Each growth factor also increased the response of the cells to gastrin, but a combination of both growth factors and gastrin did not further increase growth.
MeSH terms
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Cell Division / drug effects
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Colonic Neoplasms / drug therapy
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Colonic Neoplasms / pathology*
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Colonic Neoplasms / ultrastructure
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Drug Synergism
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Drug Therapy, Combination
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ErbB Receptors / physiology
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Gastrins / pharmacology*
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Humans
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Insulin-Like Growth Factor I / pharmacology*
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Mitogens / pharmacology
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Receptors, Cell Surface / physiology
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Receptors, Somatomedin
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Stomach Neoplasms / drug therapy
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Stomach Neoplasms / pathology*
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Stomach Neoplasms / ultrastructure
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Transforming Growth Factor alpha / pharmacology*
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Tumor Cells, Cultured
Substances
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Gastrins
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Mitogens
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Receptors, Cell Surface
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Receptors, Somatomedin
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Transforming Growth Factor alpha
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Insulin-Like Growth Factor I
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ErbB Receptors