Cell surface sialylation of three metastasizing sublines HCT116, HCT116a and HCT116b of a human colon carcinoma was shown to correlate with their in vivo tumorigenicity. Lectin binding studies revealed further differences in cell surface glycosylation between HCT116a and HCT116 sublines. Binding to collagen IV correlated with the in vivo aggressiveness of the cells, whereas binding to fibronectin did not. On a laminin substrate the most tumorigenic line adhered best, but binding of the other lines was similar. Sialidase treatment of the cells had no effect on cell binding to laminin and fibronectin, but resulted in a decrease of cell binding to collagen IV.