Prominent role for plasmacytoid dendritic cells in mucosal T cell-independent IgA induction

Hiroyuki Tezuka; Yukiko Abe; Jumpei Asano; Taku Sato; Jiajia Liu; Makoto Iwata; Toshiaki Ohteki

doi:10.1016/j.immuni.2011.02.002

Prominent role for plasmacytoid dendritic cells in mucosal T cell-independent IgA induction

Immunity. 2011 Feb 25;34(2):247-57. doi: 10.1016/j.immuni.2011.02.002. Epub 2011 Feb 17.

Authors

Hiroyuki Tezuka¹, Yukiko Abe, Jumpei Asano, Taku Sato, Jiajia Liu, Makoto Iwata, Toshiaki Ohteki

Affiliation

¹ Department of Biodefense Research, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.

PMID: 21333555
DOI: 10.1016/j.immuni.2011.02.002

Abstract

Although both conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) are present in the gut-associated lymphoid tissues (GALT), the roles of pDCs in the gut remain largely unknown. Here we show a critical role for pDCs in T cell-independent (TI) IgA production by B cells in the GALT. When pDCs of the mesenteric lymph nodes (MLNs) and Peyer's patches (PPs) (which are representative GALT) were cultured with naive B cells to induce TI IgA class switch recombination (CSR), IgA production was substantially higher than in cocultures of these cells with cDCs. IgA production was dependent on APRIL and BAFF production by pDCs. Importantly, pDC expression of APRIL and BAFF was dependent on stromal cell-derived type I IFN signaling under steady-state conditions. Our findings provide insight into the molecular basis of pDC conditioning to induce mucosal TI IgA production, which may lead to improvements in vaccination strategies and treatment for mucosal-related disorders.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adoptive Transfer
Animals
B-Cell Activating Factor / physiology
Coculture Techniques
Cytokines / physiology
Dendritic Cells / immunology*
Germ-Free Life
Immunity, Mucosal / immunology*
Immunoglobulin A, Secretory / biosynthesis*
Immunoglobulin A, Secretory / genetics
Immunoglobulin Class Switching*
Interferon Type I / physiology
Lymph Nodes / immunology
Mesentery / immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Peyer's Patches / immunology
Receptor Cross-Talk
Receptors, Antigen, T-Cell, alpha-beta / deficiency
Receptors, Antigen, T-Cell, alpha-beta / immunology
Receptors, Antigen, T-Cell, gamma-delta / deficiency
Receptors, Antigen, T-Cell, gamma-delta / immunology
Stromal Cells / immunology
Tumor Necrosis Factor Ligand Superfamily Member 13 / physiology

Substances

B-Cell Activating Factor
Cytokines
Immunoglobulin A, Secretory
Interferon Type I
Receptors, Antigen, T-Cell, alpha-beta
Receptors, Antigen, T-Cell, gamma-delta
Tnfsf13 protein, mouse
Tnfsf13b protein, mouse
Tumor Necrosis Factor Ligand Superfamily Member 13