Peripheral visceral afferents in the guinea pig were labeled by injections of wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) into the L2 and L3 dorsal root ganglia bilaterally. After anterograde transport of the tracer the following areas were examined for the presence of HRP-labeled fibers: the inferior mesenteric ganglion (IMG), the inferior mesenteric artery (IMA) with surrounding tissue, the hypogastric nerves, parts of the descending and sigmoid colon as well as the urinary bladder. Large numbers of heavily labeled fibers were found in the IMG, in the colonic nerves around the IMA and in the hypogastric nerves. In the IMG, profiles suggestive of being labeled axon terminals were observed. Labeled fibers were observed in the muscle layers of the colon and in the bladder wall. The results show that anterograde tracing with WGA-HRP can be used successfully in analyzing the morphology and structural organization of visceral afferents in the periphery.