Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9

Ranran Cheng; Jin Peng; Yonghong Yan; Peili Cao; Jiewei Wang; Chen Qiu; Lichun Tang; Di Liu; Li Tang; Jianping Jin; Xingxu Huang; Fuchu He; Pumin Zhang

doi:10.1016/j.febslet.2014.09.008

Efficient gene editing in adult mouse livers via adenoviral delivery of CRISPR/Cas9

FEBS Lett. 2014 Nov 3;588(21):3954-8. doi: 10.1016/j.febslet.2014.09.008. Epub 2014 Sep 19.

Authors

Ranran Cheng¹, Jin Peng², Yonghong Yan³, Peili Cao³, Jiewei Wang⁴, Chen Qiu³, Lichun Tang³, Di Liu³, Li Tang³, Jianping Jin⁵, Xingxu Huang⁶, Fuchu He⁷, Pumin Zhang⁸

Affiliations

¹ State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; The College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100142, China.
² State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; The Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai 200241, China.
³ State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
⁴ State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hanzhou, Zhejiang Province 310011, China.
⁵ Department of Biochemistry and Molecular Biology, University of Texas Health Sciences Center, Houston, TX 77030, United States.
⁶ Model Animal Research Center, Nanjing University, Nanjing, Jiangsu Province 210061, China.
⁷ State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China.
⁸ State Key Laboratory of Proteomics, Beijing Proteomics Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China; Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, United States. Electronic address: pzhang@bcm.edu.

PMID: 25241167
DOI: 10.1016/j.febslet.2014.09.008

Abstract

We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.

Keywords: Adenoviruses; CRISPR/Cas9; Gene editing; Liver.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviridae / genetics*
Animals
Base Sequence
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Gene Targeting / methods*
Humans
Liver / metabolism*
Male
Mice

Abstract

Publication types

MeSH terms

Grants and funding