The object of the present work was to study the influence of antral pH, of aspirin and indomethacin, and of prostaglandins A2, E1 and E2 on the intragastric output of somatostatin and gastrin induced by electrical vagal stimulation (5 V, 2 ms, 2, 5 and 10 Hz). Experiments were performed on anaesthetized rats in which the stomach was perfused with a dextran solution (pH approximately 1.5 to approximately 6). To assess the effect of vagal stimulations on gastric acid secretion and on gastrin and somatostatin release, pH as well as somatostatin and gastrin levels was recorded in the perfusate effluent. During perfusion of the stomach with dextran solution, pH approximately 6, vagal stimulation at 10 Hz decreased perfusate pH to 1.3 and gastrin and somatostatin were released at a ratio of 2:1. Less gastrin and more somatostatin (ratio 0.1:1) was released by the same stimulation when the stomach was pre-perfused with acid solution (pH approximately 1.5), although also in these experiments the pH of the perfusate fell to 1.3. When vagal stimulations were performed after the rats had been pretreated with aspirin or indomethacin, more somatostatin than gastrin was detected in the perfusate independently of the perfusate pH. The vagally induced intraluminal release of somatostatin occurring in aspirin-treated animals was abolished by a low dose of atropine (0.05 mg kg-1) or by a simultaneous infusion of prostaglandin E1 (30 micrograms kg-1 h-1).