Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA

N Tomita; Y Izumoto; A Horii; S Doi; H Yokouchi; M Ogawa; T Mori; K Matsubara

doi:10.1016/s0006-291x(89)80087-7

Molecular cloning and nucleotide sequence of human pancreatic prechymotrypsinogen cDNA

Biochem Biophys Res Commun. 1989 Jan 31;158(2):569-75. doi: 10.1016/s0006-291x(89)80087-7.

Authors

N Tomita¹, Y Izumoto, A Horii, S Doi, H Yokouchi, M Ogawa, T Mori, K Matsubara

Affiliation

¹ Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.

PMID: 2917002
DOI: 10.1016/s0006-291x(89)80087-7

Abstract

The cDNA clone encoding human prechymotrypsinogen was isolated from a human pancreas cDNA library and its nucleotide sequence was determined. The sequence consists of a 16 bp 5' non-coding region, a 789 bp amino acid coding region and a 60 bp 3' non-coding region. The predicted product consists of 263 amino acids, including 18 amino acids for a signal peptide and 15 amino acids possible for an activation peptide. Southern blot analyses using the cloned cDNA as a probe revealed that human genomic DNA carries at least two genes that are related to chymotrypsinogen.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
Chymotrypsinogen / genetics*
Cloning, Molecular
DNA / genetics
Humans
Molecular Sequence Data
Pancreas / enzymology*
Restriction Mapping
Transcription, Genetic

Substances

DNA
Chymotrypsinogen

Associated data

GENBANK/M24400