Nucleotide sequence divergence and functional constraint in VIP precursor mRNA evolution between human and rat

FEBS Lett. 1985 Apr 8;183(1):55-9. doi: 10.1016/0014-5793(85)80953-4.

Abstract

The nucleotide sequence analysis of cloned cDNA for VIP precursor from rat cerebral cortex reveals that the precursor contains both rat VIP and PHI-27. The deduced primary structure of rat VIP is identical with human VIP. The amino acid sequence of rat PHI-27 differs by 4 amino acids from human PHM-27. When each VIP precursor is divided functionally into 6 domains, the amino acid sequence homology between rat and human precursors ranges from 69 to 100%. In contrast, any domain exhibits an essentially equal degree of nucleotide sequence homology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution*
  • Cerebral Cortex / analysis
  • DNA
  • DNA, Recombinant
  • Humans
  • Male
  • Nucleic Acid Hybridization
  • Peptide PHI
  • Peptides / genetics
  • Protein Precursors / genetics*
  • RNA, Messenger*
  • Rats
  • Rats, Inbred Strains
  • Vasoactive Intestinal Peptide / genetics*

Substances

  • DNA, Recombinant
  • Peptide PHI
  • Peptides
  • Protein Precursors
  • RNA, Messenger
  • Vasoactive Intestinal Peptide
  • DNA

Associated data

  • GENBANK/X02341