Abstract
A complementary DNA encoding an amiloride-sensitive Na+ channel has been cloned and characterized from rat colon. The protein encoded by the cDNA has a sequence of 699 amino acids (79 kDa) containing several putative membrane spanning domains and potential phosphorylation sites. It forms a channel that has the electrophysiological and pharmacological properties characteristic of the epithelial Na+ channel. Homologies (including in transmembrane domains) have been found between a part of the channel sequence and the Mec4 gene product of Caenorhabditis elegans, a protein associated with mutation-induced neuronal degeneration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amiloride / pharmacology*
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Amino Acid Sequence
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Animals
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Base Sequence
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Caenorhabditis elegans / chemistry*
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans Proteins*
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Cloning, Molecular
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Colon / chemistry
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Colon / drug effects
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DNA
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Epithelial Sodium Channels
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Epithelium / chemistry
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Epithelium / drug effects
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Helminth Proteins / chemistry*
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Helminth Proteins / genetics
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics
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Molecular Sequence Data
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Phosphorylation
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Rats
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Rats, Wistar
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Sequence Homology, Amino Acid
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Sodium Channels / chemistry
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Sodium Channels / drug effects
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Sodium Channels / genetics*
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Xenopus
Substances
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Caenorhabditis elegans Proteins
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Epithelial Sodium Channels
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Helminth Proteins
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Mec-4 protein, C elegans
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Membrane Proteins
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Scnn1a protein, rat
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Sodium Channels
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Amiloride
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DNA
Associated data
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GENBANK/L03647
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GENBANK/L03648
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GENBANK/L03649
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GENBANK/L03650
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GENBANK/L03651
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GENBANK/L03652
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GENBANK/L03653
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GENBANK/L03654
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GENBANK/X70521
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GENBANK/Z19541