Abstract
A novel tumor necrosis factor (TNF) family member has been cloned and characterized. This protein, designated TNF-related apoptosis-inducing ligand (TRAIL), consists of 281 and 291 aa in the human and murine forms, respectively, which share 65% aa identity. TRAIL is a type II membrane protein, whose C-terminal extracellular domain shows clear homology to other TNF family members. TRAIL transcripts are detected in a variety of human tissues, most predominantly in spleen, lung, and prostate. The TRAIL gene is located on chromosome 3 at position 3q26, which is not close to any other known TNF ligand family members. Both full-length cell surface expressed TRAIL and picomolar concentrations of soluble TRAIL rapidly induce apoptosis in a wide variety of transformed cell lines of diverse origin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Apoptosis / drug effects*
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Apoptosis Regulatory Proteins
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Base Sequence
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Cell Line
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Chromosome Mapping
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Chromosomes, Human, Pair 3*
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Cloning, Molecular
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification*
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Humans
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / isolation & purification
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Membrane Glycoproteins / pharmacology
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Mice
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Molecular Sequence Data
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Sequence Alignment
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TNF-Related Apoptosis-Inducing Ligand
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Tumor Necrosis Factor-alpha / genetics*
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Tumor Necrosis Factor-alpha / isolation & purification
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Apoptosis Regulatory Proteins
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DNA, Complementary
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Membrane Glycoproteins
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TNF-Related Apoptosis-Inducing Ligand
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TNFSF10 protein, human
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Tnfsf10 protein, mouse
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Tumor Necrosis Factor-alpha
Associated data
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GENBANK/U37518
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GENBANK/U37522
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GENBANK/Z36726