Abstract
The cells that express Steel factor (SLF) in the gastrointestinal (GI) tract were studied using SLF-lacZ transgenic mice. Expression, detected by beta-galactosidase histochemistry, was evident in cells between the circular and longitudinal muscle layers in the GI tract. Double staining with antibodies specific for the neural markers, PGP 9.5, MAP2 and c-Ret, showed that SLF-lacZ positive cells were enteric neurons. Enteroglia did not express SLF-lacZ. The distribution of expressing cells was complimentary to the expression of c-Kit in myenteric interstitial cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Digestive System / cytology
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Digestive System / metabolism*
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Enteric Nervous System / cytology
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Enteric Nervous System / metabolism*
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Gene Expression Regulation / physiology*
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Genes, Reporter
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Histocytochemistry
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Immunohistochemistry
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Lac Operon*
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Mice
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Mice, Transgenic
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Neurons / metabolism*
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Promoter Regions, Genetic
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Proto-Oncogene Proteins c-kit / analysis
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Stem Cell Factor / genetics*
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beta-Galactosidase / analysis
Substances
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Stem Cell Factor
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Proto-Oncogene Proteins c-kit
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beta-Galactosidase