Abstract
Four exons of a human homologue of a yeast cell division cycle gene (MCM6/mis5, which is thought to encode a DNA replication licensing factor) have been identified 3.3 kb upstream from the start of transcription of the intestinal lactase gene on human chromosome 2q21, initially by similarity to a rat 'intestinal crypt-cell replication factor'. RT-PCR analysis shows, that unlike lactase, MCM6 is not restricted in its tissue distribution and does not show person-to-person variation in the level of expression in adult intestine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Amino Acid Sequence
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Cell Cycle
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Cell Cycle Proteins / biosynthesis
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics*
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Chromosome Mapping
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Chromosomes, Human, Pair 2*
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Cloning, Molecular
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DNA Replication
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Fungal Proteins / genetics*
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Gene Expression
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Genes, Fungal
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Humans
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Intestine, Small / metabolism
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Lactase
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Minichromosome Maintenance Complex Component 6
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Molecular Sequence Data
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Polymerase Chain Reaction
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Schizosaccharomyces / genetics
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Schizosaccharomyces pombe Proteins*
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beta-Galactosidase / genetics*
Substances
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Cell Cycle Proteins
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Fungal Proteins
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Schizosaccharomyces pombe Proteins
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Lactase
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beta-Galactosidase
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MCM6 protein, human
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Minichromosome Maintenance Complex Component 6
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mcm6 protein, S pombe
Associated data
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GENBANK/U67281
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GENBANK/U67282
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GENBANK/U67283
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GENBANK/U67284