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Immunoreactive gastrin components in human serum
  1. Jens F. Rehfeld,
  2. Flemming Stadil,
  3. Jørgen Vikelsøe


    The apparent molecular size and charge of immunoreactive gastrin components were studied in sera from patients with pernicious anaemia or gastrinomas (the Zollinger-Ellison syndrome) by Sephadex gel filtration and aminoethylcellulose chromatography. The following serum components were distinguished: (1) a monophasic component I similar in size to proinsulin which was converted into `little' gastrin I by trypsin digestion; (2) a biphasic component II, corresponding to `big' gastrins I and II (Gregory and Tracy); (3) a biphasic component III corresponding to `little' gastrins I and II (Gregory and Tracy); and (4) a biphasic component IV, corresponding to `minigastrins' I and II (Gregory and Tracy). `Big, big' gastrin, a plasma component found in the void volume of the Sephadex G-50 column by Yalow and Berson (1972) was undetectable in the sera investigated. A component in gastrinoma and antral mucosa extracts corresponding in size to `big big' gastrin was detectable by the assay; the `big big' gastrin fraction from gastrinoma tissue was heterogenous, with components of apparent MW 30 000-100 000. It is concluded that serum gastrin circulates in the form of at least four components, of which the three smaller ones are in pairs.

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