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K-ras gene mutation in gall bladder carcinomas and dysplasia.
  1. T Ajiki,
  2. T Fujimori,
  3. H Onoyama,
  4. M Yamamoto,
  5. S Kitazawa,
  6. S Maeda,
  7. Y Saitoh
  1. Second Department of Pathology, Kobe University School of Medicine, Japan.


    Epithelial dysplasia of gall bladder is an important precancerous lesion of gall bladder carcinogenesis. To investigate the frequency of K-ras gene mutation in gall bladder carcinoma and dysplasia, K-ras codon 12 mutations were investigated by the polymerase chain reaction/restriction enzyme based method following direct sequencing. Mutation was detected in 59% (30 of 51) of gall bladder carcinomas, in 73% (8 of 11) of gall bladder dysplasia in gall stone cases, and in 0% of the normal gall bladder epithelium. There was, however, no correlation between K-ras mutation and clinicopathological factors of gall bladder carcinoma. K-ras gene mutation occurs even in gall bladder dysplasia at an incidence similar to that in carcinomas, suggesting that testing for K-ras gene mutation may prove useful as an adjunct to bile cytological or biopsy analysis.

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