Article Text

Download PDFPDF

In vivo and in vitro hepatic phosphorus-31 magnetic resonance spectroscopy and electron microscopy in chronic ductopenic rejection of human liver allografts
Free
  1. S D Taylor-Robinsona,b,
  2. J Sargentonia,
  3. J D Bella,
  4. E L Thomasa,
  5. C D Marcusa,
  6. K K Changania,
  7. N Saeeda,
  8. H J F Hodgsonb,
  9. B R Davidsonc,
  10. A K Burroughsd,
  11. K Rollesc,
  12. C S Fostere,
  13. I J Coxa
  1. aRobert Steiner MR Unit, bGastroenterology Unit, Imperial College School of Medicine, Hammersmith Hospital, London , cUniversity Department of Surgery , dUniversity Department of Medicine, Royal Free Hospital and School of Medicine, London, eDepartment of Pathology, University of Liverpool, Liverpool, UK
  1. Dr S D Taylor-Robinson, Robert Steiner MR Unit, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK.

Abstract

Background—In vivo hepatic phosphorus-31 magnetic resonance spectroscopy (MRS) provides non-invasive information about phospholipid metabolism.

Aims—To delineate MRS abnormalities in patients with chronic ductopenic rejection (CDR) and to characterise spectral changes by in vitro MRS and electron microscopy.

Patients and methods—Sixteen liver transplant recipients (four with CDR; 12 with good graft function) and 29 controls (23 healthy volunteers; six patients with biliary duct strictures) were studied with in vivo 31P MRS. Peak area ratios of phosphomonoesters (PME) and phosphodiesters (PDE), relative to nucleotide triphosphates (NTP) were measured. In vitro MRS and electron microscopy were performed on biopsy specimens from five patients with CDR, freeze clamped at retransplantation. Phosphoethanolamine (PE), phosphocholine (PC), glycerophosphorylethanolamine (GPE), and glycerophosphorylcholine (GPC) concentrations were measured.

Results—The 12 patients with good graft function displayed no spectral abnormalities in vivo; the four patients with CDR showed significantly elevated PME:NTP (p<0.01) and PDE:NTP ratios (p<0.005). Patients with biliary strictures had significant differences in PME:NTP (p<0.01) from patients with CDR, but not in mean PDE:NTP. In vitro spectra from CDR patients showed elevated PE and PC, mirroring the in vivo changes in PME, but reduced GPE and GPC concentrations were observed, at variance with the in vivo PDE findings. On electron microscopy, there was no proliferation in hepatocyte endoplasmic reticulum.

Conclusions—The increase in PME:NTP reflects altered phospholipid metabolism in patients with CDR, while the increase in PDE:NTP may represent a significant contribution from bile phospholipid.

  • in vivo 31P magnetic resonance spectroscopy
  • in vitro 31P magnetic resonance spectroscopy
  • liver transplantation
  • chronic ductopenic rejection
  • electron microscopy
  • phospholipids
View Full Text

Statistics from Altmetric.com

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.