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Inflammation and inflammatory bowel disease
Enhanced production of monocyte chemotactic protein 3 in inflammatory bowel disease mucosa
  1. J Wedemeyera,
  2. A Lorentza,
  3. M Gökea,
  4. P N Meiera,
  5. P Flemmingb,
  6. C A Dahindenc,
  7. M P Mannsa,
  8. S C Bischoffa
  1. aDepartment of Gastroenterology and Hepatology, Medical School of Hannover, D-30623 Hannover, Germany, bDepartment of Pathology, Medical School of Hannover, D-30623 Hannover, Germany, cInstitute of Allergology and Immunology, Inselspital Bern, Bern, Switzerland
  1. Dr Bischoff.

Abstract

BACKGROUND The β chemokine monocyte chemotactic protein 3 (MCP-3) has chemoattractant and activating capabilities in monocytes, lymphocytes, eosinophils, and basophils.

AIMS To investigate MCP-3 expression in inflammatory conditions of the human intestinal mucosa.

PATIENTS Forty five colon biopsy specimens from 18 patients with inflammatory bowel disease (IBD; 16 specimens from inflamed and 10 from non-inflamed areas) and 19 control patients were examined.

METHODS Immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR) were used for MCP-3 detection in tissue sections. Intestinal epithelial cell lines (HT-29, Caco-2, T-84) were stimulated with interleukin (IL) 1β, IL-6, and tumour necrosis factor α (TNF-α) and examined for MCP-3 protein and mRNA expression using immunocytochemistry and RT-PCR, respectively.

RESULTS In tissue sections, MCP-3 protein was detected predominantly in epithelial cells, both in patients with IBD and in controls. MCP-3 staining was particularly pronounced at sites of active mucosal inflammation. The intensity of MCP-3 staining was positively correlated with the extent of epithelial destruction. In intestinal epithelial cell lines, MCP-3 mRNA was expressed, whereas MCP-3 protein was not consistently detected.

CONCLUSIONS Our data show that MCP-3 protein is present in normal and inflamed intestinal tissue. MCP-3 production is substantially enhanced in areas of active inflammation, suggesting an immunoregulatory role of MCP-3 in intestinal inflammation.

  • ulcerative colitis
  • Crohn’s disease
  • epithelial cells
  • monocyte chemotactic protein 3
  • chemokines

  • Abbreviations

    CD
    Crohn’s disease
    IBD
    inflammatory bowel disease
    IL
    interleukin
    MCP
    monocyte chemotactic protein
    MIP
    macrophage inflammatory protein
    TNF
    tumour necrosis factor
    UC
    ulcerative colitis

    • https://doi.org/10.1136/gut.44.5.629

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    • Abbreviations

      CD
      Crohn’s disease
      IBD
      inflammatory bowel disease
      IL
      interleukin
      MCP
      monocyte chemotactic protein
      MIP
      macrophage inflammatory protein
      TNF
      tumour necrosis factor
      UC
      ulcerative colitis
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