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High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 are significant target antigens of perinuclear anti-neutrophil cytoplasmic antibodies in autoimmune hepatitis
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Abstract

BACKGROUND High mobility group (HMG) non-histone chromosomal proteins HMG1 and HMG2 have been identified as novel antigens of perinuclear anti-neutrophil cytoplasmic antibodies (p-ANCAs), and the existence of anti-HMG1 and anti-HMG2 antibodies in a population of patients with ulcerative colitis has been reported.

AIMS To investigate whether HMG1 and HMG2 are target antigens for p-ANCAs in autoimmune hepatitis (AIH).

PATIENTS Serum samples from 28 patients with AIH, 44 patients with primary biliary cirrhosis (PBC), 27 patients with chronic hepatitis C, and 23 patients with chronic hepatitis B were tested.

METHODS ANCAs were detected by routine indirect immunofluorescence (IIF). Anti-HMG1 and anti-HMG2 antibodies were assayed by enzyme linked immunosorbent assay.

RESULTS p-ANCAs were detected in 89% (25/28) of patients with AIH, 36% (16/44) of patients with PBC, 11% (3/27) of patients with chronic hepatitis C, and 13% (3/23) of patients with chronic hepatitis B. Anti-HMG1 and/or anti-HMG2 antibodies were detected in 89% (25/28) of patients with AIH, 70% (31/44) with PBC, 26% (7/27) with chronic hepatitis C, and 9% (2/23) with chronic hepatitis B. In AIH, anti-HMG1 and/or anti-HMG2 antibodies were detected in 96% (24/25) of p-ANCA positive patients. The p-ANCA staining pattern detected by IIF using sera from patients with AIH disappeared or decreased in titre after preincubation with a mixture of HMG1/HMG2. The presence and titres of those antibodies in AIH correlated significantly with those of p-ANCA, but not with those of anti-nuclear antibody or anti-smooth muscle antibody.

CONCLUSIONS HMG1 and HMG2 are significant target antigens of p-ANCA in AIH.

  • perinuclear anti-neutrophil cytoplasmic antibodies
  • chromosomal proteins
  • high mobility group 1 and 2
  • autoimmune
  • hepatitis
  • Abbreviations

    ANCA
    anti-neutrophil cytoplasmic antibody
    IIF
    indirect immunofluorescence
    c-ANCA
    cytoplasmic anti-neutrophil cytoplasmic antibody
    p-ANCA
    perinuclear anti-neutrophil cytoplasmic antibody
    AIH
    autoimmune hepatitis
    PSC
    primary sclerosing cholangitis
    HMG
    high mobility group
    ELISA
    enzyme linked immunosorbent assay
    PBC
    primary biliary cirrhosis
    ANA
    anti-nuclear antibody
    SMA
    anti-smooth muscle antibody
    AMA
    anti-mitochondrial antibody
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  • Abbreviations

    ANCA
    anti-neutrophil cytoplasmic antibody
    IIF
    indirect immunofluorescence
    c-ANCA
    cytoplasmic anti-neutrophil cytoplasmic antibody
    p-ANCA
    perinuclear anti-neutrophil cytoplasmic antibody
    AIH
    autoimmune hepatitis
    PSC
    primary sclerosing cholangitis
    HMG
    high mobility group
    ELISA
    enzyme linked immunosorbent assay
    PBC
    primary biliary cirrhosis
    ANA
    anti-nuclear antibody
    SMA
    anti-smooth muscle antibody
    AMA
    anti-mitochondrial antibody
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