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Differential expression of cyclooxygenase 2 in human colorectal cancer
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Abstract

BACKGROUND Experimental, clinical, and epidemiological studies have implicated mitogenic metabolites of arachidonic acid such as prostaglandin E2(PGE2) in colorectal carcinogenesis. Recently, cyclooxygenase 2 (COX-2) which catalyses the conversion of arachidonic acid to PGE2, has displayed increased levels in human colorectal cancer.

AIMS To evaluate whether there is differential COX-2 expression from different locations (caecum, ascending, transverse, descending, or sigmoid colon, and rectum) in human colorectal cancer.

METHODS Protein levels of COX-2 were determined by western blot analysis in tumours and adjacent normal mucosa of 39 patients with colorectal cancer.

RESULTS There was a notable overexpression of COX-2 protein in tumours located in the rectum (p<0.001) compared with other locations in the colon. Rectal tumours revealed elevated COX-2 protein levels in 18/20 cases compared with 4/19 colonic cases. No association between enhanced COX-2 protein expression in tumour tissue and Dukes’s stages was found.

CONCLUSIONS Results suggest that the differential COX-2 expression may be due to differences in gene regulatory factors affecting COX-2 expression and/or reflect secondary changes in tumour progression which may have clinical implications.

  • cyclooxygenase 2
  • protein expression
  • human colorectal cancer
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Footnotes

  • Abbreviations used in this paper:
    APC
    adenomatous polyposis coli
    COX
    cyclooxygenase
    cPLA2
    cytosolic phospholipase A2
    NSAID
    non-steroidal anti-inflammatory drug
    PGE2
    prostaglandin E2
    PLA2-II
    group II phospholipase A2
    PPARγ
    peroxisome proliferator activated receptor
    TCF/LEF
    T cell factor/leucocyte enhancing factor

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